昆仑雪菊中多种成分的含量测定

目的:测定昆仑雪菊中多种成分的含量.方法:总黄酮采用紫外分光光度法；总皂苷采用重量法；砷的测定采用二乙基二硫代氨基甲酸银分光光度法；汞的测定采用冷原子吸收分光光度法；铅的测定采用石墨炉原子分光光度法；微量元素采用硝酸作为消解液,利用微波消解的方法处理,原子吸收分光光度法测定；氨基酸采用比色法,比较各种菊花中的多种成分含量.结果:昆仑雪菊中的蛋白质含量为13,32％,总糖26.81％,总黄酮11.6％,总皂苷111.44 mg·g-1,总氨基酸9.83％.结论:经过检测,昆仑雪菊中蛋白质、糖类和总黄酮含量高于其他菊花,为其今后的开发利用提供基础.

Determination of Ingredients in Coreop Sistinctoria

Objective: to determine the content of diversified ingredients in Coreop sistinctoria. Method: Using ultraviolet spectrophotometry to examine the flavonoids; the total saponins adopt the weight method; arsenic determination use silver diethyl dithio carbamate spectrophotometry; the determination of hydrargyric use cold atomic absorption spectrophotometry; the determination of lead use graphite furnace atomic spectrophotometry;the determination of trace elements using nitric acid liquid as solvent,than manager it using microwave ultrasound method,and under the atomic absorption spectrophotometry examine it; amino acids by colorimetric method, comparing all kinds of component content of various kinds of Coreop sistinctoria useing Amino acids colorimetric method. Result: The protein content of Coreop sistinctoria is 13.32%, the total sugar is 26.81%, the flavonoids is 11.6% and the total saponins is 111.44 mg·g^-1, the total amino acids is 9.83%. Conclusion: After detection, the protein, carbohydrate and the flavonoids content of Coreop sistinctoria is higher than other chrysanthemum, it provides a foundation for us in future use and developmentit.