HPLC同时测定山茱萸总苷分散片中莫诺苷和马钱苷的血浆浓度

目的:建立HPLC法同时测定大鼠血浆中莫诺苷和马钱苷含量的方法,并研究山茱萸总苷分散片在大鼠血浆和各组织器官中的药动学行为.方法:Diamonsil C18色谱柱,甲醇-水梯度洗脱,流速1 mL· min-1,检测波长237 nm,血浆和各组织样品采用甲醇直接沉淀蛋白的方法.结果:血浆中莫诺苷和马钱苷的线性范围分别是0.612 ～ 30.6,0.921～46.0 mg· L-1；日内与日间RSD＜ 15％,回收率在90.2％ ～111.6％.大鼠单次灌胃山茱萸总苷分散片后,在血浆、胃和小肠中检测到莫诺苷和马钱苷.结论:该方法简便、快速、重复性好,适用于莫诺苷和马钱苷血药浓度测定及药动学研究.

Simultaneous Determination of Morroniside and Loganin in Rats Plasma from Dispersible Tablets of Total Glucosides from Cornus officinalis by HPLC

Objective: To set up an HPLC method for determination of morroniside and loganin in rat plasma, and study the pharmacokinetics of dispersible tablets of total glucosides from Cornus officinalis in rat plasma and tissues. Method: The procedure of HPLC was performed on a column of Diamonsil C₁₈, taking methanol-water as mobile phase in gradient elution. The flow rate was 1 mL·min^-1 and detection wavelength was 237 nm. The samples of plasma and tissues were prepared based on a simple protein precipitation with methanol. Result: The linear ranges of morroniside and loganin were, respectively, from 0.612 to 30.6 mg·L^-1, and from 0.921 to 46.0 mg·L^-1. The RSD values of intra-day and inter-day were less than 15%. The recovery was between 90.2% to 111.6%. After dispersible tablets of total glucosides from C. officinalis was administered orally, morroniside and loganin were found in plasma, stomach and small intestine samples. Conclusion: The method is simple and quick with a good repeatability, which can be used in the determination of plasma concentration of morroniside and loganin and pharmacokinetic study.