主成分及聚类分析法对不同产地头花蓼的综合质量评价

目的:综合比较、评价不同产地头花蓼的质量.方法:采用高效液相色谱法、紫外分光光度法、水溶性浸出物测定法,对不同产地的41个野生及栽培头花蓼中槲皮素、槲皮苷、没食子酸、总黄酮、浸出物进行测定,并以上述5个指标成分的含量为指标,运用主成分和聚类分析对不同产地的41个野生及栽培头花蓼进行综合质量评价研究.结果:不同产地头花蓼中槲皮素、槲皮苷、没食子酸、浸出物、总黄酮含量有明显差异,除贵州省毕节县亮岩镇2号样品外,其余样品均达到药用要求.以贵州东部头花蓼药材样品的综合质量较好.结论:头花蓼样品中所含的上述化学成分受产地、土壤类型影响较大,受海拔影响较小.其中,贵州西部地区头花蓼野生资源较为丰富,但其指标性成分槲皮素含量及综合质量低于贵州东部地区及外省样品；贵州东部地区头花蓼槲皮素含量较高、综合质量较好,验证了头花蓼基地建在黔东南施秉的合理性.

Comprehensive Quality Evaluation of Polygonum Capitatum from Different Habitats by Principal Component Analysis and Cluster Analysis Method

Objective: To campare and evaluate the quality of Polygonum capitatum from different habitats. Method: Quercetin, quercitrin, gallic acid, general flavone and extractives of 41 wild and cultivated P. capitatum were determined by HPLC,US and water soluble extractive method.Through the content of five indicated ingredients as the index,the quality of 41 wild and cultivated P. capitatum was assessed by principal componentanalysis and cluster analysis method. Result: The differeces of quercetin, quercitrin, gallic acid, general flavone and extractives among P. capitatum from different habitats are significant. In addition to sample 2 from town of Liangyan Bijie County, the remaining samples are up to the medical requirements.The quality of P. capitatum in east of guizhou is the best. Conclusion: The chemical composition above in P. capitatum is more influenced by the place of origin and the type of soil comparatively than by the elevation.The wild P. capitatum in the west guizhou is widespread,but the content of the indicated ingredient-quercetin-is lower than the east one and other provinces, so reaches the same conclusion to the comprehensive quality. The content and the comprehensive quality of quercetin of P. capitatum in the east guizhou is better.,which proves the rationality of GAP base of P. capitatum in the southeast guizhou.This study build up the reference to the quality,excellent provenance selection and GAP production of P. capitatum.