HPLC测定截叶铁扫帚不同药用部位中槲皮素、山奈酚的含量

目的:建立高效液相色谱法同时测定截叶铁扫帚不同药用部位中槲皮素、山奈酚含量的方法.方法:采用Daisogel Sp-ODS-BP C18色谱柱(4.6 mm×250 mm,5 μm),流动相甲醇-0.2％磷酸(63∶37),流速1.0 mL·min-1,柱温25℃,检测波长360nm.结果:槲皮素在6.25～100 mg·L -呈良好的线性关系(r =0.999 9),加样回收率100.75％,RSD 1.92％,山奈酚在0.638～20.4 mg·L -呈良好的线性关系(r =0.999 9),加样回收率为99.76％,RSD 1.99％.根、枝、叶部位槲皮素的平均质量分数分别为9.00,41.79,221.86 μg·g-1,山奈酚的平均质量分数分别为3.09,7.52,40.72 μg·g-1.结论:该方法简便快速,结果准确可靠,可作为截叶铁扫帚不同药用部位的含量测定方法,为合理开发利用截叶铁扫帚资源提供理论依据.

Determination of the Content of Quercetin and Kaempferol from Different Medicinal Parts of Lespedeza cuneata by HPLC

Objective:To develop a HPLC method for the determination of the content of quercetin and Kaempferol from different medicinal parts of Lespedeza cuneata.Method: Samples were analyzed on Daisogel Sp-ODS-BP C18(4.6 mm×250 mm,5 μm),with the mobile phase consisted of methanol-0.20% phosphoric acid solution(63∶37).The flow rate was 1.0 mL ·min-1,column tomparature was at 25 ℃.The UV wavelength was set at 360 nm.Result:The linear range of quercetin was 6.25-100 mg·L-1.The average recovery was 100.75% and RSD was 1.92%).The linear range of kaempferol was 0.638-20.4 mg·L-1.The average recovery was 99.76% and RSD was 1.99%.The concent of quercetin in root,branch and leaf was 9.00,41.79,221.86 μg·g-1.The concent of kaempferol was 3.09,7.52,40.72 μg·g-1.Conclusion: The method is simple,rapid,accurate and reliable.It can be used as a content determination method for the medicinal parts of L.cuneata.It can provide evidence for the rational development and utilization of L.cuneata.