| 多重聚合酶链反应对2006年上海地区呼吸道病毒感染的病原学检测分析 |
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| 引用本文： | 周明哲,宋志刚,胡芸文.多重聚合酶链反应对2006年上海地区呼吸道病毒感染的病原学检测分析[J].世界感染杂志,2009,9(3):157-159. |
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| 作者姓名： | 周明哲 宋志刚 胡芸文 |
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| 作者单位： | 上海市（复旦大学附属）公共卫生临床中心科研中心,上海201508 |
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| 基金项目： | 上海市卫生局青年科研项目（054Y19） |
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| 摘 要： | 目的探讨2006年上海地区急性呼吸道病毒感染的病原学分布。方法建立并优化同时检测流感HIN1亚型、流感H3N2亚型、流感B（Flu B）型、呼吸道合胞体病毒（RSV）的多重聚合酶链反应（Multiplex PCR）方法，并对2006年上海地区112份呼吸道感染患者标本进行检测。结果多重PCR方法能在7h内完成检测，特异性较好；112份样品中共检出H3N2 12株，Flu B 5株，RSV1株，总检测率为16．1％。结论初步建立能同时检测4种病毒的多重PCR方法；上海地区2006年流感H3N2亚型呈散发流行。
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| 关 键 词： | 急性呼吸道感染 流感 呼吸道合胞病毒 Multiplex PCR |
Etiology detection of Shanghai respiroviru infection status in 2006 by multiplex PCR |
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| Institution: | ZHOU Ming-zhe, SONG Zhi-gang, HU Yan-wen (Research center, Shanghai public health clinical center affiliated to Fudan University, Shanghai 201508, China) |
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| Abstract: | Objective To investigate the virus distribution of acute respiratory infection in Shanghai in 2006. Methods A multiplex PCR method was developed and optimized by which Influenza virus subtype H1N1, H3N2, Influenza virus B and respiratory syncytial virus （RSV） can be simultaneously identified, and with this method 112 specimens from Shanghai hospitals were analyzed. Results The method is capable to fulfill the detection within 7 hours with a high specificity. The total detecting rate was 16.2% （H3N2 10.7%, Flu B 4.5%, RSV 1%）. Conclusion A rapid, specific multiplex PCR method for the simultaneous detection has been primarily studied. The Influenza virus subtype H3N2 is predominant in acute respiratory infection in Shanghai in 2006. |
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| Keywords: | Respiratory virus Multiplex PCR |
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