| 兔脊髓缺血再灌注损伤与正常组蛋白质组的差异分析 |
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| 引用本文： | 朱本清,杨小玉,顾锐,邱宾涛.兔脊髓缺血再灌注损伤与正常组蛋白质组的差异分析[J].中国实验诊断学,2010,14(4):487-489. |
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| 作者姓名： | 朱本清 杨小玉 顾锐 邱宾涛 |
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| 作者单位： | 1. 吉林大学中日联谊医院,骨科,吉林,长春,130033
2. 北京蛋白质组中心,北京,112206 |
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| 基金项目： | 国家自然基金资助项目: |
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| 摘 要： | 目的建立脊髓缺血再灌注损伤双向凝胶电泳图谱,对照观察蛋白质的差异表达。方法建立脊髓缺血再灌注损伤模型,获取损伤和正常脊髓的蛋白质组双向电泳荧光图谱,观察差异蛋白的不同时间点的变化规律,建立缺血再灌注24 h与正常脊髓蛋白质组的匹配差异图谱,鉴定差异蛋白。结果成功建立损伤和正常的双向电泳荧光图谱和肽质量指纹图谱,比较再灌注损伤24 h组与正常组可检测到46个蛋白差异点,鉴定出10个差异蛋白。结论损伤24 h组较其他组变化明显,与正常脊髓的蛋白质组存在明显差异。
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| 关 键 词： | 脊髓 缺血再灌注损伤 蛋白质组 双向电泳 质谱鉴定 |
Difference in proteome Maps between spinal cord ischemia reperfusion injury (IR) and normal rabbit spinal cord |
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| ZHU Ben-qing,YANG Xiao-yu,GU Rui,et al..Difference in proteome Maps between spinal cord ischemia reperfusion injury (IR) and normal rabbit spinal cord[J].Chinese Journal of Laboratory Diagnosis,2010,14(4):487-489. |
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| Authors: | ZHU Ben-qing YANG Xiao-yu GU Rui |
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| Institution: | ZHU Ben-qing,YANG Xiao-yu,GU Rui,et al.(Department of Orthopaedics China Japanese Friendship Hospital,Jilin University,Changchun 130033,China) |
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| Abstract: | Objective To establish two dimensional electrophoresis(2DE) gels of spinal cord ischemia reperfusion injury(IR) of rabbit and analyze differential expressions of proteins in spinal cord.Methods To establish animal model of IR and obtain DIGE of IR and normal.The rule of the protein dots change with time to observed.The images analyses and establishment of the matching differentially displayed map between injured and normal rabbit spinal cord,separate and identification the proteome from protein in 2DE.Resul... |
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| Keywords: | spinal cord ischemia reperfusion injury(IR) proteome two dimensional electrophoresis mass identified |
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