通心络胶囊减轻再灌注诱导的家兔心肌细胞凋亡效应的研究

目的观察通心络胶囊的抗心肌细胞凋亡效应,并探讨凋亡相关基因蛋白Bcl-2和Bax在其中的作用.方法制备在体兔心肌缺血/再灌注模型,并随机分成3组,观察各组心肌梗死范围、心肌细胞凋亡以及Bcl-2和Bax的表达.用氯化三苯基四氮唑(TTC)确定心肌梗死范围.心肌细胞凋亡采用末端标记法(TUNEL)和DNA琼脂糖凝胶电泳(DNA Laddering)检测,Bcl-2和Bax的表达用原位免疫组化检测.结果通心络组能明显缩小心肌梗死范围,凋亡细胞较生理盐水组稀疏,生理盐水组梗死周边心肌组织DNA呈云梯状条带,通心络组则较为整齐.结论通心络可抑制再灌注诱导的心肌细胞凋亡,抑制Bcl-2、Bax的表达.

Tongxinluo Attenuates Reperfusion-induced Myocyte Apoptosis in Rabbit Heart

Objective To study the hypothesis that the reduction of myocardial infarct size caused by Tongxinluo(TXL) is associated with inhibition of apoptotic myocyte death by modulating expression of anti-apoptotic Bcl-2 and pro-apoptotic Bax proteins.Methods In anesthetized rabbits the left anterior descending coronary artery was occluded. All experimental animals were divided into three groups randomly. The effects of TXL on myocardial infarct size, cardiomyocyte apoptosis, Bcl-2 and Bax proteins were observed in rabbit hearts. Infarct size was measured by triphenyltetrazolium chloride (TTC) staining. Cardiomyocyte apoptosis was detected by histological TUNEL staining and confirmed by DNA ladder on agarogel electrophoresis. The expression of Bcl-2 and Bax proteins was determined by immunohistochemical staining in situ.Results TXL decreased infarct size significantly; TUNEL positive cells in the peri-infarct zone of the ischemic myocardium were significantly reduced in the Ischemia/Reperfusion(I/R) group; DNA Laddering pattern was revealed in I/R group while it was not found in TXL group; TXL inhibited the expression of Bcl-2 and Bax.Conclusion It is suggested that TXL inhibits cardiomyocyte apoptosis induced by reperfusion. The down-regulation of Bcl-2 and Bax plays an important role in the anti-apoptotic effect of TXL.