幽门螺杆菌粪便抗原与血清学抗体联合检测在儿童幽门螺杆菌感染流行病学调查中的应用

目的 评价幽门螺杆菌粪便抗原(HpSA)检测与传统血清学检测方法联合应用在儿童Hp感染流行病学调查中的可行性。方法 无症状儿童480例行Hp血清学抗体检测。对Hp血清学阳性儿童再行粪便HpSA检测。以HpSA阳性作为Hp现症感染诊断标准;以血清抗Hp-IgG抗体阴性作为未感染的诊断标准。以Hp现症感染儿童作为病例组,未感染儿童作为对照组,探讨儿童Hp感染危险因素。结果 血清抗Hp抗体阳性率为35.8％(172／480)。HpSA阳性率即Hp现症感染率为13.3％(64／480)。儿童Hp感染呈家庭内聚集及口-口传播和粪-口传播途径。结论 HpSA原与血清学检测两种非侵入性方法相结合简便、易行、费用相对低廉、准确性较高,适合在儿童中开展Hp感染流行病学调查。

Combined application of helicobacter pylori stool antigen test and serology in epidemiologic study of helicobacter pylori infection in children

Objective To evaluate the feasibility of combined application of helicobacter pylori stool antigen(HpSA)-and traditional serology in epidemiologic study of helicobacter pylori(Hp) infection in children. Methods Hp serum IgG antibodies were examined among 480 healthy children. Heticobacter pylori stool antigen(HpSA) were measured among children who were seropositive. Hp status was defined as positive when stool antigen test was positive, Hp status was defined as negative when seronegative. A case - control study was conducted to identify risk factors of Hp infection in preschool children. Results Among 480 preschool children, 172 (35 8 %) were sero- positive, 64(13.3 %) with positive result of HpSA test were diagnosed as current Hp infection. Univariate factor analysis demonstrated that Hp infection clustered in families and Hp infection was often acquired in childhood by oral-oral and fecal - oral person to person transmission. Conclusions HpSA and serology screening are two nomnvasive tests, simple not costly, effective and accurate. The combination of these 2 noninvasive methods can be applied in epidemiologic study of Hp infection in childrenJAppl Clin Pediatr,2004,19(11):948-950