| 高效液相色谱法测定黄体酮自微乳化胶囊的含量 |
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| 引用本文： | 杨向平,王文喜.高效液相色谱法测定黄体酮自微乳化胶囊的含量[J].中国药业,2009,18(10):31-32. |
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| 作者姓名： | 杨向平 王文喜 |
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| 作者单位： | 1. 浙江省人民医院,浙江,杭州,310024
2. 浙江工业大学药学院,浙江,杭州,310032 |
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| 摘 要： | 目的建立高效液相色谱（HPLC）法测定黄体酮自微乳化胶囊的含量。方法采用依利特C18柱（150mm×4．6mm，5μm），流动相为甲醇-水（75：25），流速为1．0mL／min，内标物为丙酸睾丸素，检测波长为254nm。结果黄体酮质量浓度在0．01048—0．2620mg／mL范围内与峰面积线性关系良好（r=0．9997），平均回收率为100．03％，RSD为1．34％（n=5）。结论HPLC法操作简便易行，结果准确可靠，可用于黄体酮自微乳化胶囊的含量测定。
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| 关 键 词： | 高效液相色谱法 黄体酮 内标法 |
Determination of Progesterone Self-Microemulsfying Capsule by HPLC |
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| Yang Xiangping,Wang Wenxi.Determination of Progesterone Self-Microemulsfying Capsule by HPLC[J].China Pharmaceuticals,2009,18(10):31-32. |
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| Authors: | Yang Xiangping Wang Wenxi |
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| Institution: | 1. Zhejiang Provincial People＇s Hospital, Hangzhou, Zhejiang, China 310024; 2. College of Pharmacy, Zhejiang University of Technology, Hangzhou, Zhejiang, China 310032) |
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| Abstract: | Objective To establish a HPLC method for the content determination of Progesterone Self- Microemulsfying Capsule. Methods HPLC was carried out using the Elitte C18 column （150 mm×4.6 mm, 5 μm） and the mobile phase consisting of methanol-water（75：25） with the flow rate of 1.0 mL/min. The internal standard was testosterone propionate, and the wavelength was 254 nm. Results The standard curve was linear over the range of 0. 010 48-0. 262 0 mg/mL for progesterone with r=0. 999 7. The recovery rate for progesterone was 100.03% and RSD was 1.34% （n=5）. Conclusion The proposed method is simple, efficient and accurate, which can be used for the content determination of Progesterone Self-Microemulsfying Capsule. |
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| Keywords: | HPLC progesterone internal standard method |
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