氧氟沙星联用抗结核药肝毒性的实验研究

目的 :研究氧氟沙星联用抗结核药的肝毒性并探讨其机制。方法 :分别测定异烟肼组、利福平组、异烟肼利福平联合用药 (IR)组及其联用氧氟沙星 (IRO)组小鼠的血清谷氨酸氨基转移酶 (ALT)、肝指数、肝匀浆丙二醛(MDA)含量、肝微粒体细胞色素p45 0和线粒线Ca2 + ATP酶活性 ,及肝病理检查 ,结果与对照组以及实验组之间进行比较。结果 :与正常对照组及单用异烟肼、利福平组比较 ,IR组及IRO组的血清ALT、肝细胞匀浆MDA明显增高 (P <0 0 5 ) ;同时发现IR组和IRO组的肝微粒体细胞色素p45 0含量较高和线粒体Ca2 + ATP酶活性较低 ;病理检查IRO和IRC组肝细胞坏死程度稍轻但与IR组比较无明显差异。结论 :异烟肼和利福平联用氧氟沙星肝毒性增加 ,但并不比异烟肼和利福平联合时强。

Experimental study on hepatic toxic injury induced by ofloxacin with Antituberculosis drugs in mice

Objective: To study hepatic toxic injury induced by ofloxacin with Antituberculosis drugs in mice. Methods: Thelevel of serum glutamic-pyruvic transaminase(ALT), liver index, content of malondialdehyde (MDA) of liver homogenate, activity of hepatic microsome enzyme and mitochondrial membrane Ca2 + -ATP enzyme,and degree of liver cell injury were investigated in mice which are in control group and the experimental groups of isoniazid ( I ), rifampicin (R), combining isoniazidwith rifampicin(IR), combining ofloxacin with isoniazid and rifampicin(IRO). Results: The groups of IR, IRO could markedlyincrease the level of ALT, and content of MDA of liver homogenate ( P < 0.05) and decrease the activity of mitochondrialmembrane Ca2 +-ATP enzyme ( P > 0.05),but IRC doesn＇t induced these alterations. IR, IRO could increase the degree of liver injury,but there was no difference among the groups of IR,IRO. Conclusion: Combination ofloxacin with isoniazid and rifampicin in mice increase hepatic toxic injury, but the level of injury was little lower than combination isoniazid with rifampicin.