大蒜素对大鼠NK细胞肿瘤杀伤活性的影响

目的: 研究大蒜素对体外培养的大鼠NK细胞肿瘤杀伤活性的影响,并初步分析其可能机制。方法: 免疫磁珠分选法分离大鼠脾脏NK细胞,流式细胞仪检测不同浓度的大蒜素对NK细胞的增殖和凋亡的影响,ELISA检测大鼠脾脏NK细胞IFN-γ的分泌水平,乳酸脱氢酶(LDH)法检测大鼠脾脏NK细胞对小鼠淋巴瘤Yac-1细胞的杀伤活性。结果: 大蒜素对体外培养的NK细胞有明显的促进增殖和抑制NK细胞自然凋亡的作用,并能提高NK细胞分泌IFN-γ的水平和增强对Yac-1细胞的杀伤毒性,且在一定的浓度范围内呈剂量依赖性,其中30 mg/L是大蒜素的相对适宜浓度。结论: 大蒜素可能通过上调NK细胞分泌IFN-γ的水平,增强其体外肿瘤杀伤能力。

Effects of allicin on anti-tumor activity of rat NK cells in vitro

AIM: To investigate the role of allicin on the anti-tumor activity of rat NK cells in vitro. METHODS: CD3^-NKR-P1⁺ NK cells were isolated from rat spleen using Dynabeads FlowComp^TM Flexi system. The proliferation and apoptosis of the NK cells induced by allicin were analyzed by the method of flow cytometry. The levels of IFN-γ in the supernatants were measured by ELISA. Lactate dehydrogenase release assay was used to detect the cytotoxicity of the NK cells.RESULTS: In vitro, allicin significantly promoted the proliferation of NK cells, inhibited NK cell apoptosis, increased the level of IFN-Fγ in supernatants and enhanced the cytotoxicity of the NK cells to Yac-1 tumor cells in a dose-dependent manner. Allicin at concentration of 30 mg/L exerted the maximal effect on activating NK cells. CONCLUSION: Allicin enhances the activity of NK cells in vitro by upregulating IFN-γ expression.