RP-HPLC法测定开心散中人参皂苷Rg1和Re的含量

【目的】建立高效液相色谱法（RP-HPLC）测定开心散中有效成分人参皂苷Rg1和Re含量的方法。【方法】采用Eclipse XDB-C18色谱柱（4．6×250mm，5μm），美国Agilent公司；流动相：乙腈-0．05％磷酸水（19：81）；流速：1．0ml／min；柱温：25℃；检测波长：203nm。【结果】人参皂苷Rg1在0．0011～0．0176mg呈良好线性关系（r=0．9999，n=5），平均回收率为96．07％，RSD为0．77％（n=3）；人参皂苷Re在0．0005～0．0080mg呈良好线性关系（r=0．9999，n=5），平均回收率为98．98％，RSD为1．46％（n=3）。【结论】用本法测定开心散中人参皂苷含量，操作简便，结果准确，且可同时对人参皂苷Rg1及Re进行测定。此法可作为开心散有效部位的质量控制方法之一。

Determintion of Ginsenoside Rg1 and Re in Kaixinsan by RP-HPLC

Objective] To establish a RP-HPLC method for quantitative determination of ginsonoside agl and Re in Kaixinsan. Methods] Eclipse XDB-C18 column （4.6 × 250 mm, 5 μm） was used.The mobil phase was acetonnitrile-0.05% phosphoric acid solution （19： 81）; flow rate was 1.0 ml/min; column temperature： 25 %; the detecting wavelength was 203nm. Resuits] The calibration curves of ginsenoside Rg1 was linear in the range 0.0011 - 0.0166 mg （ r = 0.9999, n = 5 ）, the average recoveries was 96.07 %, RSD = 0.77 % （ n = 3） ; the calibration curves of ginsenoside Re was linear in the range 0.0005 -0.0080 mg （ r =0.9999, n = 5）, the average recoveries was 98.98%, RSD= 1.46% （ n = 3） . Conclusions] This method is convenient and exact for determining the content of ginsenoside in Kaixinsan, and it can determine Rg1 and Re in the same time.