丹参对增生性瘢痕成纤维细胞形态的影响及增殖抑制初探

目的探索丹参治疗增生性瘢痕的机理。方法将培养的增生性瘢痕成纤维细胞加入含丹参的培养液中培养２４ｈ，与未加药者对照比较。结果①含丹参组成纤维细胞由长梭形变为圆形，而培养上清中的乳酸脱氢酶（ＬＤＨ）活性及ＭＴＴ（四甲基偶氮唑）比色法结果，用药组与对照组均无显著差异（Ｐ＞０．０５）；②流式细胞仪检测各时相细胞ＤＮＡ水平：用药组Ｇ０＋Ｇ１期细胞的百分比明显高于对照组（Ｐ＜０．０１），而Ｇ２＋Ｍ期百分比则显著低于对照组（Ｐ＜０．０１）。结论丹参能改变增生性瘢痕成纤维细胞形态，而不影响其活力并抑制其增殖。

Effects of Salviae Miltiorrhizae on the morphology of hypertrophic scar fibroblast and initial exploration on the inhibition of proliferation

Objective To explore the possible mechanism of Salviae Miltiorrhizae (SM) in the treatment of hypertophic scar (HS). Methods Put the cultured HS fibroblasts derived from patients into the culture fluid containing SM for 24 hours, and then compare with the control group without SM. Results The spindleshape cells were discovered becoming retraction and round. Lactate dehydrogenase (LDH) activity and MTT assays showed no significant difference between the two groups ( P 5BZ>0.05). The percentage of the DNA level in the G0 G1 phase, analyzed by the means of flowing cytometry, was higher than in the control group (P <0.01), while in the G2 M phase the percentage in the former group was lower than the control group( P <0.01).THZConclusion The SM can cause the alteration in cell morphology of HS fibroblast without damaging its viability, and also can exert an inhibitory effect on fibroblast proliferation.