Establishment of red fluorescent protein-tagged HeLa tumor metastasis models: determination of DsRed2 insertion effects and comparison of metastatic patterns after subcutaneous,intraperitoneal, or intravenous injection |
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Authors: | Lu Jau-Yeong Chen Hui-Chun Chu Ricky Yuan-Yuan Lin Tsu-Chun Emma Hsu Ping-I Huang Ming-Shyan Tseng Ching-Jiunn Hsiao Michael |
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Institution: | (1) Department of Internal Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan;(2) Department of Radiation Oncology, Kaohsiung Chang-Gung Memorial Hospital, Kaohsiung, Taiwan;(3) Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan;(4) Department of Internal Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan |
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Abstract: | Metastasis is the leading cause of death in patients with cervical cancer. In this report, we establish novel fluorescent
HeLa tumor metastasis models to determine whether HeLa transfected with the enhanced red fluorescent protein (DsRed2) gene
in vitro and xenotransplanted through subcutaneous, intraperitoneal, or intravenous route into SCID mice would permit the detection
of tumor micro-metastasis in vivo. Our results showed that DsRed2 insertions did not interfere the tumorigenic properties of HeLa cells. We also demonstrated
that DsRed2-transduced HeLa cells maintained stable high-level DsRed2 expressions during their growth in vivo. DsRed2 fluorescence clearly demarcated the primary seeding place and readily allowed for the visualization of distant micro-metastasis
and local invasion at the single-cell level. Lung metastasis, the major cause of cervical carcinoma related death, was found
in all three models. However, intravenous injections of the HeLa-DsRed2 cells established tumor foci in the lung, while subcutaneous
and intraperitoneal injections only established lung metastasis at single-cell levels. The DsRed2 tagged HeLa cancer model
allowed detection and investigation of physiologically relevant patterns of cancer invasion and metastasis in vivo.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Cervical carcinoma DsRed2 metastasis model SCID mice |
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