荷瘤裸鼠肌肉内基因电转染对移植瘤的作用

目的　以人甲状腺未分化癌细胞系TA K建立的人甲状腺未分化癌裸鼠皮下移植模型为对象 ,研究裸鼠肌肉内电转染对移植瘤的作用。方法　将皮下移植肿瘤的裸鼠分为 5组 :空白对照组、pcDNA 3质粒肌肉电转染组、TIMP 3质粒肌肉注射组、TIMP 3质粒肌肉电转染组及TIMP 3质粒肿瘤电转染组。肌肉电转染采用的参数是电场强度为 2 0 0V/cm ,脉冲时值为 2 0ms ,8次 ,1Hz;肿瘤内电转染采用的参数是电场强度为 6 0 0V/cm ,脉冲时值为 2 0ms,1次 ,1Hz。结果　TIMP 3质粒肌肉内电转染组和TIMP 3质粒肿瘤内电转染组肿瘤生长受到抑制 ,与另外 3组相比肿瘤生长速度明显减缓 (P<0 .0 5 ) ,且两组肿瘤组织中TIMP 3蛋白量过度表达。结论　抑癌基因可通过肌肉内DNA电转染起到抗肿瘤效果。

Effects of DNA Electrotransfer in Muscle on the Implanted Tumor Growth in Nude Mice

Objective To study the antitumor effects of DNA electrotransfer in muscle (ETM) by using established animal model for human anaplastic thyroid cancer cell line TA K.Methods Nude mice with implanted TA K were divided into five groups including: control group, pcDNA 3 plasmid electrotransfered into muscle (pcDNA ETM group), TIMP 3 plasmid injected into muscle (TIMP 3 IM group), TIMP 3 plasmid electrotransfered into muscle (TIMP 3 ETM group), TIMP 3 plasmid electrotransfered into implanted tumor (TIMP 3 ETT group). Electrical parameters used in electrotransfer were: 200 V/cm, 20 ms/pulse; 8 pulses, 1 Hz in muscle and 600 V/cm, 20 ms/pulse; 1 pulses, 1 Hz in implanted tumor respectively. Results The growth of TA K was inhibited more significantly in the groups of TIMP 3 plasmid electrotransfered into muscle and TIMP 3 plasmid electrotransfered into implanted tumor than the other groups ( P <0.05), and the content of TIMP 3 protein in tumor tissues was higher in these two groups . Conclusion Anti oncogene has the antitumor effects by DNA electrotransfer in muscle.