反义核酸抑制放疗诱导的肝癌细胞VEGF高表达

目的：探讨放疗过程中放疗患者血清血管内皮生长因子（VEGF）升高的机理，以及VEGF反义硫代寡核苷酸（Antisense oligodeoxynucleotide,AS-ODNs）对肝癌细胞VEGF表达分泌的抑制作用。方法。动态观察肿瘤患者放疗期间血清VEGF变化，体外培养肿瘤细胞，观察放疗对肿瘤细胞VEGF表达分泌的诱导作用及反义寡核苷酸的抑制效应。结果：肿瘤患者血清VEGF浓度在放疗期开始后逐渐升高，1－2周达到峰值后开始下降，到第6周时基本恢复到正常，肝癌细胞受X射线照射后VEGF的分泌量较对照组明显升高，VEGF反核组癌细胞VEGF蛋白表达水平较单纯照射组显著下降（P＜0．01）。结论：放射可诱导肿瘤细胞中VEGF的表达分泌。放疗患者放疗过程中血清VEGF浓度升高可能是由于癌细胞受照射后VEGF表达，分泌增加所致，VEGF反义寡核苷酸对放疗诱导的VEGF高表达有较好的抑制作用。

Inhibitory action of antisense oligodeoxynucleotide on radiotherapy-induced expression of vascular endothelial growth factor(VEGF) in a liver cancer cell line

Objective To study the mechanism of serum VEGF increase in tumor patients during radiotherapy and the inhibitory action of its antisense oligodeoxynucleotide (AS|ODN) on expression of VEGF protein induced by radiotherapy in a liver cancer cell line. Methods To detect the change of the serum VEGF in tumor patients during radiotherapy dynamically and the inhibitory action of the (AS|ODN) on expression of VEGF induced by radiotheraphy in a liver cancer cell line. Results The changes of the serum VEGF in four patients receiving radiotherapy with watched continuously.Their levels of serum VEGF began to increase during radiotherapy with the peak value appeared in the first two weeks,and then declined to the pre|radiotherapy range.Irradiating the liver cancer cells with X|rays significantly increased the VEGF expression and secretion.The level of VEGF protein in the patient group treated with AS|ODN and X irradiation was significantly than that of the group treated with X irradiation only. Conclusion The induction of VEGF protein expression by X irradiation in tumor cells may result in increase of the serum VEGF in the tumor patients during radiotherapy and the induction can be blocked by VEGF AS|ODN.;