正常供者外周血造血干细胞动员效果及影响因素

 目的　探讨粒细胞集落刺激因子（G-CSF）动员正常供者外周血造血干细胞的效果、毒副作用及影响因素。方法　对59名异基因造血干细胞正常供者采用G-CSF 皮下注射3 ~ 5 d，使用COBE Spectra血细胞分离机采集外周血干细胞，流式细胞术检测采集物中CD+34细胞数。结果　所有供者第一次采集的单个核细胞（MNC）及CD+34细胞量平均值分别为4.4（1.12～13.06）×108／kg供者体重及3.78（1.14～12.92）×106／kg供者体重。患者不良反应轻微。男性供者、年龄小于45岁者及采集前白细胞计数高者采集所得CD+34细胞数较高。结论　G-CSF作为正常供者动员剂安全有效。患者性别、年龄及采集前白细胞计数可作为预测因素。

G-CSF mobilization of peripheral blood hematopoietic stem cells in normal healthy donors

Objective　To study the efficacy, safety and predictable factors of G-CSF-based mobiliza－tion in normal healthy donors for allogeneic peripheral blood stem cell (PBSC) transplantation. Methods　G-CSF was used to mobilize PBSCs in 59 healthy donors and PBSCs were collected by COBE Spectra cell sepa－rator. CD＋34 cells were calculated by flow cytometry. Results　The numbers of mononucleated cells (MNCs) and CD＋34 cells of the first collection were 4.4(1.12~13.06)×108 and 3.78(1.14~12.92)×106 per kilogram donor weight, respectively. The side effects were mild. Significantly more total number of CD＋34 cells were mobilized in men, < 45 years old or those with higher leukocyte counts before apheresis. Conclusion　G-CSF mobiliza－tion of PBSCs in normal healthy donors is effective and safe. Sex, age or leukocyte counts before apheresis can predict CD＋34 cell collections.