| 重组空肠弯曲菌蛋白疫苗免疫 BA LB／c小鼠的研究 |
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| 引用本文： | 刘琳琳,;任志国,;王法权,;姚远,;孙运芳,;张西强.重组空肠弯曲菌蛋白疫苗免疫 BA LB／c小鼠的研究[J].山东医学高等专科学校学报,2014(2):107-109. |
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| 作者姓名： | 刘琳琳 ;任志国 ;王法权 ;姚远 ;孙运芳 ;张西强 |
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| 作者单位： | [1]山东医学高等专科学校,山东临沂276000; [2]山东医学高等专科学校附属医院,山东临沂276000 |
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| 基金项目： | 山东省高等学校发展计划项目（No.J12LK14）;临沂市科技发展计划项目（No.201313063） |
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| 摘 要： | 目的探讨重组空肠弯曲菌rLTB-PEB1蛋白疫苗的免疫机制。方法经口服免疫 BALB/c 小鼠后，ELISA 法检测特异性抗体IgG 、IgA 水平；ELISPOT 法检测派伊尔结特异性抗体分泌细胞；实时荧光定量PCR 检测IFN-γ、IL-4 mRNA 表达的差异。计算疫苗保护率。结果免疫组小鼠派伊尔结特异性抗体、IFN-γ、IL-4 mRNA 水平明显增高（ P ＜0．05）。免疫组的疫苗保护率为89．3％～93．7％，可维持30周。结论重组空肠弯曲菌rLTB-PEB1蛋白疫苗口服免疫小鼠可有效预防空肠弯曲菌的感染，其免疫机制可能为诱导了TH1/TH2型免疫应答。
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| 关 键 词： | 空肠弯曲菌 蛋白疫苗 BALB c小鼠 疫苗保护率 |
EXPERIMENT ON BALB/CMICE WITH RECOMBINANT PROTEIN VACCINE |
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| Institution: | LIU Lin-lin , REN Zhi-guo , WANG Fa-quan , et al (Shandong Medical College, Linyi, China 276000) |
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| Abstract: | Objective To study the immunological response induced by Campylobacter jejuni （CJ） recom-binant protein vaccine .Methods After the oral immunization for BALB/c mice ,the levels of IgG and IgA were detected by ELISA , the specific antibody secreting cells in Payer’s patch were detected by ELISPOT , the expression levels of IFN-γ and IL-4 were detected by RT-PCR .The vaccine protection rate was calculated .Results The levels of specific antibody , IFN-γ and IL-4 were increased obviously after immuni-zation .The vaccine protection rate was 89 .3%-93 .7%（ P 〈 0 .05） .Conclusion The oral immunization with recombinant protein vaccine may induce TH1/TH2 type immune responses and can produce protective im-munity against Campylobacter jejuni . |
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| Keywords: | Campylobacter jejuni Protein vaccine BALB/c mouse Vaccine protection rate |
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