| 兔下颌骨髁状突软骨细胞体外培养及生物学特性的研究 |
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| 引用本文： | 李松,沈丽宁,税艳青,吕冰. 兔下颌骨髁状突软骨细胞体外培养及生物学特性的研究[J]. 昆明医学院学报, 2000, 21(4): 21-24 |
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| 作者姓名： | 李松 沈丽宁 税艳青 吕冰 |
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| 作者单位： | 昆明医学院口腔系,昆明 650031 |
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| 基金项目： | 云南省应用基础研究基金项目(99C0008R) |
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| 摘 要： | 为探讨兔下颌骨髁状突软骨细胞的体外培养方法及其生物学特性，用胰酶及胶原酶联合消化法获取软骨细胞，并在ＤＭＥＭ培养基中进行原代及传代培养；通过形态学观察及免疫组织化学染色对软骨细胞的贴壁率、生长曲线及表型等细胞生物学特性进行了研究。结果：所获软骨细胞主呈多角形，第３代软骨细胞１２ｈ贴壁率达９５％，软骨细胞倍增时间约５５．１ｈ，６代以内软骨细胞的Ⅱ型胶原蛋白稳定表达。结果表明：本方法获软骨细胞具有稳定
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| 关 键 词： | 颞下颌关节 细胞培养 下颌骨 髁状突软骨细胞 |
| 文章编号： | 1003-4706(2000)04-0021-04 |
| 修稿时间： | 2000-10-28 |
The Study of Biological Behaviors of Rabbit Condylar Chondrocytes in Vitro |
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| LI Song,SHEN Li-ning,SHUI Yan-qin,LU Bin. The Study of Biological Behaviors of Rabbit Condylar Chondrocytes in Vitro[J]. Journal of Kunming Medical College, 2000, 21(4): 21-24 |
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| Authors: | LI Song SHEN Li-ning SHUI Yan-qin LU Bin |
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| Abstract: | To investigate the biological behaviors of rabbit condylar chondrocytes, the primary chondrocytes were harvested from newborn rabbits by dissection and sequential digestion with 0 25% trypsin and 0 l % collagenase IA The isolated chondrocytes were cultured in DMEM medium, their morphology, growth and phenotype were observed by phase-contrast microscope and immunohistochemical method Results: The chondrocytes displayed polygonal dominated and the positive result of collagen 11 within six passage Conclusion: The chondrocytes can kept steady proliferation and their phenotype in this culture condition |
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| Keywords: | Temporomandibularjoint Chondrocyte Cell culture |
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