重组人内皮抑素对肺鳞癌细胞抑制作用的体外实验

 目的 探讨重组人内皮抑素（恩度）对肺鳞状细胞癌细胞系H-520细胞生长的影响及其机制。 方法 应用四甲基偶氮唑盐（MTT）法检测恩度不同浓度和作用时间对H-520细胞的生长抑制作用；Hoechst33258染色观察恩度处理后 细胞凋亡形态并通过流式细胞术对凋亡细胞进行定量及检测细胞周期分布。采用划痕修复实验检测内皮抑素处理后H-520细胞的 迁移能力。 结果 恩度可以抑制H-520细胞的生长，且呈时间-剂量依赖性；恩度可以促进H-520细胞的凋亡，随时间延长，凋亡率显著增加，与 对照组相比，差异具有统计学意义（P<0.01）；恩度可以引起H-520细胞周期分布变化，细胞发生G₀/G₁ 期阻滞。划痕实验表明，恩度可以使H-520细胞体外迁移能力显著下降，与对照组相比，24h划痕修复率差异具有显著意义。 结论 恩度对H-520细胞增殖具有明显抑制作用；其主要机制为促进细胞凋亡，调整细胞周期分布和减弱细胞迁移能力。

Inhibitory Effects of Lung Squamous Cell Carcinoma in Vitro by Endostar

Objective To investigate the inhibitory effect of endostar on human lung squamous cell carcinoma cell line H-520 and to reveal the possible mechanism of the inhibition. Methods The effect of endostar with various concentrations on the proliferation of H-520 cell was studied by MTT assay. Hoechest33258 staining was used for morphological examination and flow cytometry was used to detect the apoptosis and cell cycle distribution. Wound healing assay was used to evaluate the migration of H-520 cell after administration of endostar. Results Endostar inhibited H-520 cell proliferation with time dose dependent style and induced cell apoptosis,the apoptosis rate was increased with the raise of endostar concentration(P<0.01).Flow cytometric analysis showed that cell cycle distribution changed and G₀/G₁ arrest occurred after exposure to endostar. Wound healing assay indicated that cells after administration of endostar were far less motile than control groups. The wound healing ratio after 24h between the endostar exposure and controls was significantly different. Conclusion Endostar inhibits proliferation of H-520 cells by inducing apoptosis,changing the cell cycle distribution and attenuating cell migration.