雷公藤甲素对体外培养大鼠气道平滑肌细胞增殖及原癌基因的影响

 目的探讨雷公藤甲素(triptolide,TL)对培养的大鼠气道平滑肌细胞(ASMC)增生及原癌基因c-fos与c-jun表达的影响。方法按不同药物分为6组，TL组(浓度分别为5，20，80，320，1 280 μg·L_-1)及地塞米松1 mg·L_-1组，采用MTT法检测不同浓度药物对ASMC增殖的影响，同时进行逆转录-聚合酶链反应(RT-PCR)检测各组c-fos，c-jun 的 mRNA表达水平。结果MTT方法显示TL对ASMC增殖有明显抑制作用，并呈剂量依赖性，TL的IC₅₀为51.52 mg·L_-1；RT-PCR方法显示TL浓度分别为80，320，1 280 μg·L_-1和地塞米松1 mg·L_-1能显著抑制c-fos，c-jun mRNA表达。结论TL有抑制ASMC增生的作用，其机制可能与下调c-fos和c-jun表达有关。

Study on effects of triptolide on proliferation of airway smooth muscle cells and expression of oncogene in vitro

OBJECTIVE To investigate the effects of triptolide on airway smooth muscle cells (ASMC) proliferation and expressions of oncogene c-fos and c-jun in culturing ASMC.METHOD S The primary ASMC from rat were collected and cultured and studied.The effects of triptolide at different doses (5,20,80,320 and 1 280 μg·L_-1) and dexamethasone (1 mg·L_-1) on ASMC proliferation were studied by MTT method.The mRNA expressions of c-fos and c-jun form ASMC were examined by RT-PCR.RESULTS The ASMC proliferation was inhibited at the dose (IC₅₀=51.52 mg·L_-1).The mRNA expression of c-fos and c-jun in ASMC was also inhibited by triptolide (80,320 and 1 280 μg·L_-1) and dexamethasone.CONCLUSION S It suggested that triptolide remarkably inhibited ASMC proliferation probably by suppressing the expression of c-fos and c-jun.