球囊损伤对动脉表皮生长因子受体表达的影响

目的；观察球囊损伤对动脉平滑肌细胞（ＳＭＣ）表皮生长因子受体（ＥＧＦＲ）表达的影响，探讨ＥＧＦＲ与再狭窄的关系。方法：于兔右颈总动脉球囊损伤前及损伤后２４ｈ、４８ｈ、７２ｈ、７天和１４天，取实验动脉段用免疫组化ＬＳＡＢ法检测ＥＧＦＲ的表达。结果：正常叫动脉中膜未见ＥＧＦＲ阳性表达，动脉球囊损伤后２４ｈ中膜部分ＳＭＣ显示ＥＧＦＲ阳性表达。４８ｈ中膜呈ＥＧＦＲ阳性表达的ＳＭＣ较２４ｈ增加，７２ｈ又较４

Effect of balloon injury on expression of epidermal growth factor receptor in artery

Purpose To observe the effect of balloon injury on the expression of epidermal growth factor receptor (EGFR) in arterial smooth muscle cells (SMC) and to study the relationship between EGFR and restenosis. Methods Rabbit carotid arteries were harvested before and 24 h, 48 h, 72 h, 7 d, 14 d after balloon injury and expression of EGFR was detected by immunohistochemical LSAB method. Results Normal artery showed no EGFR expression, but 24 h after balloon injury, some of SMC exhibited EGFR expression in medial layer, expression of EGFR increased 48 h postinjury and further increased 72 h postinjury. Expression of EGFR significantly decreased in medial layer 7 d and 14 d postinjury, and they were much less than that 72 h postinjury. But there were large amount of EGFR expression in proliferating SMC of neointima 7 d and 14 d postinjury. Positive staining were located mainly on cellular membrance. Conclusion The balloon injury to artery induces the experssion of EGFR in the injury loci and EGFR expression is associated with SMC proliferation during restenosis.