HPLC法测定龙胆中龙胆苦苷的含量 |
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引用本文: | 李馨,王金宏,金迪,江蔚新. HPLC法测定龙胆中龙胆苦苷的含量[J]. 黑龙江医药, 2010, 23(3): 319-320 |
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作者姓名: | 李馨 王金宏 金迪 江蔚新 |
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作者单位: | 哈尔滨商业大学 |
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基金项目: | 哈尔滨商业大学研究生基金 |
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摘 要: | 目的:采用HPLC法测定(Agilent Eclipse XDB—C18柱,150mm×4.6mm,5um)龙胆中龙胆苦苷的含量。方法采用高效液相色谱法;C18色谱柱(流动相:甲醇-水(25:75)流速:1ml/min;检测波长:270nm;柱温:25℃;进样量:10ul。结果:龙胆苦苷在0.1mg/mL-1.0mg/mL之间线性良好。得回归方程,Y=309.72X-16.788,r=0.9998。,测得龙胆苦苷平均加样回收率为99.38%。KSD为0.62%。结论:本法快速简便,准确,重现性好。
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关 键 词: | 龙胆 龙胆苦苷 含量测定 |
Determination of Gentiopierin in Gentian by HPLC |
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Affiliation: | Li Xin, et al (Harbin Commerce University (Harbin 150076)) |
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Abstract: | Objective: To establish a HPLC method for the determination of gentiopicfin in gentian. Methods: HPLC method, the analytical column was C 18(150 mm × 4.6 mm, 5 um).The mobile phase consisted of methanol - water (25:75) .The flow rate was 1.0 ml/min. The column temperature was at 25℃.The Detector wavelength was set at 270 nm. The injection volume was 10 ul.Results:The calibration curve was linear in the range of 0. 1 - 1.00 ug/ml of gentiopicrin with correlation coefficient of 0.9998,and the regression equation as Y=309.72X-16.788.The average recovery rate was 99.38%,and RSD was 0.62%.Conclusion: The method is quick, accurate, and simple with good repetition. |
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Keywords: | Gentian Gentiopicrin Content determination |
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