Prevention of CCl4-induced nephrotoxicity with Sonchus asper in rat |
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| Authors: | Rahmat Ali Khan Muhammad Rashid Khan Sumaira Sahreen Jasia Bokhari |
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| Affiliation: | 1. Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri–734013, West Bengal, India;2. Molecular Cytogenetics Laboratory, Department of Botany, University of North Bengal, Siliguri–734013, West Bengal, India;3. Chemical Signal and Lipidomics Laboratory, Department of Botany, University of Calcutta, Kolkata 700019, India;4. Department of Pathology, North Bengal Medical College, West Bengal, India;1. Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences (SLU), Box 7011, 750 07 Uppsala, Sweden;2. Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, India;3. Natural Products Research Centre, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;4. Department of Pharmacology, Taipei Medical University, No. 250, Wu-hsing Street, Taipei 110, Taiwan;5. Department of Food Science and Food Chemistry, Swedish University of Agricultural Sciences, Uppsala 756 51, Sweden |
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| Abstract: | Sonchus asper (SA) is locally used in renal aliments. The present work investigated the antioxidant effects of S. asper methanolic extract (SAME) against CCl4-induced nephrotoxicity in Sprague–Dawley male rats. CCl4 (3 ml/kg b.w., i.p.; 30% in olive oil) biweekly for 4 weeks induced lipid peroxidation, as reflected by significant increase of TBARS; diminished the renal antioxidant defenses, as revealed by a decrease of the level of GSH, CAT, SOD, GST, GSR, GSH-Px and QR while elevated the level of γ-GT, H2O2 and nitrite contents. CCl4 caused histopathological injuries and significantly increased the renal AgNORs count and DNA damage. Telomerase activity in kidney was determined positive with CCl4 treatment. Creatinine, urobilinogen and urea concentration was increased whereas creatinine clearance was decreased in serum and urine. Level of protein and albumin was increased in urine while reduced in serum. Serum level of nitrite was increased with CCl4 treatment. Treatment of rats with SAME (100, 200 mg/kg b.w.) effectively ameliorated the alterations induced with CCl4 in lipid peroxidation, antioxidant defenses, biochemical markers, genotoxicity and renal lesions. The present data suggests that SAME protect the kidneys possibly by alleviating the oxidative stress induced with CCl4 in rat. |
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