Fenvalerate inhibits the growth of primary cultured rat preantral ovarian follicles |
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| Authors: | Juan Fei Jian-Hua Qu Xin-Liang Ding Kai Xue Chun-Cheng Lu Jian-Feng Chen Ling Song Yan-Kai Xia Shou-Lin Wang Xin-Ru Wang |
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| Affiliation: | 1. Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029, China;2. Center of Clinical Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;1. Department of Surgery, Yale University School of Medicine, New Haven, CT;2. Department of Surgery, Bridgeport Hospital, Yale New Haven Health, Bridgeport, CT;1. State Key Laboratories for AgroBiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China;2. College of Basic Medical Sciences, Dalian Medical University, Dalian, PR China;3. College of Life Sciences, Peking University, Beijing, PR China;4. National Animal Genetic Research Center, Beijing, PR China;5. Department of Animal Science, Oklahoma State University, Stillwater, OK, USA;1. Southern California Coastal Water Research Project, Costa Mesa, CA, USA;2. University of California at San Diego, La Jolla, CA, USA;3. Department of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy;4. University of California at Riverside, Riverside, CA, USA;5. California State University at Long Beach, Long Beach, CA, USA;6. Southern Nevada Water Authority, Las Vegas, NV, USA;7. City of San Diego Public Utilities Department, San Diego, CA, USA;8. City of Los Angeles, Environmental Monitoring Division, Playa del Rey, CA, USA;9. San Diego State University, San Diego, CA, USA |
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| Abstract: | Fenvalerate is a widely used synthetic pyrethroid insecticide and is reported to disrupt reproductive function in humans and animals. However, little is known about its influence on follicular development. In this study, rat preantral follicles were primary cultured to investigate the effects of fenvalerate on follicular survival rate, morphological change, steroid hormone levels and steroidogenesis related gene mRNA expression. Follicles were cultured with 0, 1, 5 and 25 μmol/L fenvalerate for 72 h. And then the morphous was assessed by conventional light microscopy, steroid hormones were measured by RIA, and the expressions of steroidogenic acute regulatory protein (StAR) and cytochrome P450 side-chain cleavage enzyme (P450scc) were monitored by real-time quantitative PCR analysis. Results showed that fenvalerate inhibited the augmentation of follicular diameters but did not have detectable effects on follicular survival rates. The level of steroid hormones, such as progesterone, testosterone and estradiol, was inhibited. The inhibition might be due to the decreased expression levels of StAR and P450scc. These results suggested that fenvalerate restrained the follicular growth, and inhibited steroidogenesis by reducing StAR and P450scc gene expression, which might further contribute to the fenvalerate-induced reproductive dysfunction. |
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