大鼠烫伤早期肠黏膜组织热休克蛋白HSP70和HSP90的表达

目的 研究烧伤早期肠黏膜组织热休克蛋白HSP70和HSP90的表达、组织含量和分布的变化规律，探讨烧伤早期肠黏膜组织细胞的热休克反应在机体的病理生理反应中的意义。方法 以烫伤大鼠为模型，利用ELISA、免疫印迹分析和免疫组织化学方法，分析和研究肠黏膜组织HSP70和HSP90的表达、组织含量和分布及其功能状态。结果 (1)大鼠烫伤早期肠黏膜游离HSP70的含量有非常显的短暂降低；(2)肠黏膜组织HSP70和HSP90的总体含量在烧伤后有显升高；(3)烧伤早期肠黏膜组织HSP70的分子结构存在显的不均一性。结论 肠黏膜组织细胞中两种热休克蛋白表达、含量和分布的规律性变化，在肠黏膜组织细胞的应激反应、进而在肠道的黏膜屏障机制中，可能有重要的意义。

The significance of the expression of the HSP70 and HSP90 in the intestinal mucosa in scalded rats during early postburn stage

Objective To investigate the changes in the expression, the tissue contents and the distribution of heat shock protein (HSP)70 and HSP90 in the intestinal mucosa in scalded rats during early postburn stage and to elucidate the effects of heat shock response of intestinal mucosal cell on burn victim's systemic pathophysiology. Methods The scalded rats were taken as the model. ELISA, immunoblotting analysis and immunohistochemical methods were employed to analyze and study the expression, the tissue contents and the distribution and their functional status of HSP70 and HSP90 in the intestinal mucosa. Results (1)The free HSP70 content in the intestinal mucosa exhibited significantly transient decrease in the early postburn stage. (2) The total contents of HSP70 and HSP90 in the intestinal mucosa increased obviously postburn. (3)There exhibited remarkable heterogeneity of the molecular structure of HSP70 in the intestinal mucosa in the early postburn stage. Conclusion The expression and the changes in the contents and the tissue distribution of the two HSPs in the intestinal mucosal cell might play important roles in the cellular stress reaction of the intestinal mucosa and even in the intestinal barrier functional mechanism