广枣黄酮槲皮素和山萘酚促人白血病HL-60细胞系凋亡及其机制探讨

本研究旨在探讨广枣黄酮主要成份槲皮素及山萘酚的抗白血病作用及其可能的作用机制。采用MTT法测定槲皮素及山萘酚对HL-60细胞的增殖抑制作用；用流式细胞术检测药物对HL-60细胞周期的影响及其诱导凋亡作用；通过Western blot观察药物诱导的HL-60细胞凋亡中survivin蛋白表达情况。结果表明，槲皮素及山萘酚对HL-60细胞的增长有显著的抑制作用，并呈浓度和时间依赖性（槲皮素相关系数r=0．77，山萘酚相关系数r=0．76）。给药后HL-60细胞发生G2／M期阻滞和凋亡。药物诱导HL-60细胞survivin蛋白表达下调。结论：广枣黄酮的主要成份槲皮素及山萘酚有明显的抗白血病作用，且槲皮素诱导凋亡作用强于山萘酚。其作用机制可能是通过抑制细胞增殖、细胞周期阻滞、抑制survivin蛋白而诱导细胞凋亡。

Apoptosis-inducing Effect of Quercetin and Kaempferol on Human HL-60 Cells and Its Mechanism

The purpose of this study was to explore the anti-leukemie effect of quercetin and kaempferol and its mechanism. The HL-60 cells were used as the leukemia models. The inhibitory effects of quercetin and kaempferol on growth of HL-60 cells was assessed by MTT assay. The effect of guercetin and kaempferol on cell cycle in HL-60 cells was detected by flow cytometry. The cytotoxic effect of these 2 drugs was analysed by single cell electrophoresis assay. Western blot analysis was used to study the apoptotic mechanism of HL-60 cells. The results showed that the quercetin and kaempferol had a significant anti-leukemia effect in vitro. The proliferation of HL-60 cells was significantly inhibited in dose-and time-dependent manners after treating with quercetin （ r = 0.77 ） and kaempferol （ r = 0.76 ） respectively, and the cytotoxicity of quercetin was superior to that of kaempferol. The quercetin and kaempferol induced G2/M arrest and apoptosis of HL-60 cells. The quercetin and kaempferol could down-regulate the survivin expression. It is concluded that the quercetin and kaempferol have significant anti-leukemia effect in vitro. Furthermore the apoptosis-inducing effect of quercetin is stonger than that of kaempferol, both of which induce apoptosis of HL-60 cells through depressring cell growth, arresting cell cycle and inhibiting expression of survivin.