肾细胞癌组织中内皮抑素、MVD表达变化及意义

目的检测肾细胞癌（RCC）组织中内皮抑素的表达与肿瘤微血管密度（MVD）,并探讨其临床意义。方法采用免疫组化SABC法检测50例RCC组织及30例正常肾组织中的内皮抑素及CD34。结果正常肾脏组织中内皮抑素阳性表达率为36.7%,RCC组织中为76%,二者相比,P〈0.01。内皮抑素在Ⅲ~Ⅳ期RCC中的表达情况与Ⅰ~Ⅱ期RCC相比,P〈0.05。RCC组织中的MVD均值为（35.62±11.38）条/HP,明显高于正常肾脏组织的（18.71±9.53）条/HP,P〈0.01。Ⅲ~Ⅳ期RCC组织中的MVD为（41.29±10.65）条/HP,显著高于Ⅰ~Ⅱ期的（24.74±7.83）条/HP,P〈0.01。RCC组织中的Endostatin与MVD有相关性（P〈0.05）。结论 RCC组织中内皮抑素表达上调,MVD升高。二者可作为评估RCC恶性程度、转移及预后的重要指标。

Expression and signifilance of endostation and MVD in renal cell carcinoma

Objective To study the expression of endostatin and tumor microvessel density（MVD） in renal cell carcinoma（RCC） and their clinical significance.Methods With anti-endostatin McAb（multiclone antibody） and anti-CD34 McAb,immunohistochemistry was used to study the expression of endostatin and the tumor MVD in 50 cases of RCC and in 30 cases of normal renal tissue as controls.Results The expression ratios of endostatin in normal kidney tissue and in renal cell carcinoma were 37 and 76% respectively（χ2=14.892,P0.01）.The endostatin expression in 50 cases of carcinoma was associated with clinical stages（P0.05）,while no significant difference was detected in sex,age,the size of tumor and histologic grade.The mean MVD in renal cell carcinoma and in normal tissue was（35.62±11.38） and（18.71±9.53） respectively（t=6.824,P0.01）.In 50 cases of carcinoma,the mean MVD in stage Ⅲ～Ⅳ（41.29±10.65） was much higher than in stageⅠ～Ⅱ（24.74±7.83）,P0.01.There were statistically significant correlation between endostatin and MVD（P0.05）.Conclusions Endostatin and MVD may play a role in the biological behavior of RCC and may serve as an important indicator of malignancy,distant metastasis and prognosis of RCC.