Local origin and activity-dependent generation of nestin-expressing protoplasmic astrocytes in CA1 |
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Authors: | Golo Kronenberg Li-Ping Wang Martine Geraerts Harish Babu Michael Synowitz Paloma Vicens Gudrun Lutsch Rainer Glass Masahiro Yamaguchi Veerle Baekelandt Zeger Debyser Helmut Kettenmann Gerd Kempermann |
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Institution: | 1. Max Delbrück Center for Molecular Medicine (MDC), Berlin-Buch, Robert-R?ssle-Str. 10, 13125, Berlin, Germany 2. Department of Psychiatry, Charité University Medicine, Berlin, Germany 3. Division of Molecular Medicine, Department of Molecular and Cellular Medicine, Katholieke Universiteit Leuven, Leuven, Belgium 4. Department of Neurosurgery, HELIOS Hospital Berlin-Buch, Berlin, Germany 5. Department of Psychology, Rovira & Virgili University, Ctra Valls s/n, 43007, Tarragona, Spain 6. Department of Physiology, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan 7. Volkswagenstiftung Research Group, Department of Experimental Neurology, Charité University Medicine Berlin, Berlin, Germany
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Abstract: | Since reports that precursor cells in the adult subventricular zone (SVZ) contribute to regenerative neuro- and gliogenesis
in CA1, we wondered whether a similar route of migration might also exist under physiological conditions. Permanent labeling
of SVZ precursor cells with a lentiviral vector for green fluorescent protein did not reveal any migration from the SVZ into
CA1 in the intact murine brain. However, in a nestin-GFP reporter mouse we found proliferating cells within the corpus callosum/alveus
region expressing nestin and glial fibrillary acidic protein similar to precursor cells in the neighboring neurogenic region
of the adult dentate gyrus. Within 3 weeks of BrdU administration, BrdU-positive nestin-GFP-expressing protoplasmic astrocytes
emerged in CA1. Similar to precursor cells isolated from the dentate gyrus and the SVZ, nestin-GFP-expressing cells from corpus
callosum/alveus were self-renewing and multipotent in vitro, whereas cells isolated from CA1 were not. Nestin-GFP-expressing
cells in CA1 differentiated into postmitotic astrocytes characterized by S100β expression. No new neurons were found in CA1.
The number of nestin-GFP-expressing astrocytes in CA1 was increased by environmental enrichment. We conclude that astrogenesis
in CA1 is influenced by environmental conditions. However, SVZ precursor cells do not contribute to physiological cellular
plasticity in CA1. |
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Keywords: | Nestin Neurogenesis Alveus Migration Gliogenesis Stem cell |
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