| 血小板衍化牛长因子对人牙髓成纤维细胞DNA和胶原蛋白合成的影响 |
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| 引用本文： | 陈强 史俊南 李波. 血小板衍化牛长因子对人牙髓成纤维细胞DNA和胶原蛋白合成的影响[J]. 牙体牙髓牙周病学杂志, 2004, 14(1): 7-9 |
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| 作者姓名： | 陈强 史俊南 李波 |
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| 作者单位： | [1]第四军医大学口腔医学院，陕西西安710032 [2]第四军医大学生物技术中心，陕西西安710032 |
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| 摘 要： | 目的：观察血小板衍化生长因子（platelet-derived growth factor,PDGF）对人牙髓成纤维细胞（human dental pulp fibroblast,HDPF）DNA合成和胶原蛋白合成的影响。方法：应用^3H-TdR和^3H-脯氨酸掺入方法，观察PDGF对体外培养的HDPF DNA合成和胶原蛋白合成的情况。结果：20-60ng/mL PDGF可明显促进HDPF DNA的合成，40ng/mL浓度使细胞DNA合成在36h达最大值；对细胞的胶原蛋白合成无明显促进作用。结论：PDGF明显促进HDPF DNA的合成，可能在治疗牙髓病中起重要作用。
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| 关 键 词： | 血小板衍化生长因子 人牙髓成纤维细胞 DNA合成 胶原蛋白合成 |
| 文章编号： | 1005-2593(2004)01-0007-03 |
| 修稿时间： | 2003-10-13 |
The effects of platelet-derived growth factor on stimulating DNA and collagen protein synthesis of human dental pulp fibroblasts |
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| CHEN Qiang,SHI Jun-nan,LI Bo. The effects of platelet-derived growth factor on stimulating DNA and collagen protein synthesis of human dental pulp fibroblasts[J]. Chinese Journal of Conservative Dentistry, 2004, 14(1): 7-9 |
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| Authors: | CHEN Qiang SHI Jun-nan LI Bo |
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| Abstract: | AIM:DNA and collagen protein synthesis of human dental pulp fibroblasts (HDPF)were determined by treating with platelet-derived growth factor(PDGF).METHODS:HDPF were cultured with PDGF and DNA and collagen protein synthesis were measured by 3H-TdR and 3H-proline incorporation.RESULTS:PDGF significantly accelerated DNA synthesis of HDPF in dose-dependent manner over a concentration range of 20 ng/mL to 60 ng/mL ,with a maximal response at a concentration of 40 ng/mL at 36 h. PDGF did not stimulate the collage protein synthesis of HDPF.CONCLUSIN:PDGF promotes DNA synthesis of HDPF dependently, and may play an important role in pulp therapy. |
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| Keywords: | platelet-derived growth factor human dental pulp fibroblast DNA synthesis collagen protein synthesis |
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