人参二醇组皂苷对内毒素休克大鼠脑皮质TLR4 mRNA表达的影响

目的：通过观察内毒素休克大鼠脑皮质中NOS活性、NO含量和TLR4 mRNA的表达及人参二醇组皂苷（PDS）对其的影响，探讨内毒素引起脑组织损伤的分子机制。 方法： 大鼠随机分为实验对照（control）组、内毒素休克（LPS）组、地塞米松（LPS+Dex）组和人参二醇组皂苷（LPS+PDS）组。大鼠静脉注射内毒素（4 mg/kg）4 h后测定脑组织中NOS活性、NO含量及TLR4 mRNA的表达。 结果： LPS+Dex组和LPS+PDS组NOS活性、NO2-/NO3-含量显著低于LPS组（P＜0.05），TLR4 mRNA表达亦明显低于LPS组。 结论： PDS能够下调脑组织中TLR4 mRNA的表达，降低NOS活性、NO含量，对中枢神经系统具有保护作用。

Effects of PDS on TLR4 mRNA expression in cortex in endotoxic shock rats

AIM: To explore the molecular mechanism of brain tissue injury induced by endotoxin. METHODS: Rats were divided into LPS, LPS+DEX, LPS-PDS and control group, respectively. NOS activity, NO content and TLR4 mRNA expression were assayed 4 h after intravenous injection of LPS. RESULTS: NOS activity, NO content and TLR4 mRNA expression in LPS + DEX and LPS + PDS groups were obviously lower than those in LPS group. CONCLUSION: PDS may provide protective effects on the central nervous system by down - regulating TLR4 expression, reducing NOS activity and NO content in the brain tissue.