肝郁脾虚证多发性抽动症大鼠模型的建立及评价

目的:建立肝郁脾虚证多发性抽动症大鼠动物模型,初步了解该模型的一般特征。方法:亚氨基二丙腈(IDPN)腹腔注射诱导法后,用饮食失节、慢性束缚以及过度疲劳法建立多发性抽动症肝郁脾虚证大鼠模型。通过自主活动计数、刻板运动评分、体重、食量、胸腺及脾脏指数、胃泌素、D-木糖等指标对肝郁脾虚证多发性抽动症大鼠进行评价。结果:模型组、肝郁脾虚组大鼠造模后在相同的时间内活动路程及平均速度较空白对照组明显增加。肝郁脾虚组大鼠的进食量减少,胸腺与脾脏指数,胃泌素及D-木糖浓度差异均有统计学意义(P 0. 05)。结论:本模型有儿童多发性抽动症的动作性抽动和发声性抽动的症状,同时具有小儿多发性抽动症中医证型中的肝郁脾虚证中情志抑郁,神疲气短,少气懒言,食少便溏等相似症状。动物模型的表面效度方面具有较大的相似程度。

Establishment and Preliminary Evaluation of Rats Model of Tourette's Syndrome (TS) with Liver Stagnation and Spleen Deficiency Syndrome

To establish rats model of tourette''s syndrome (TS) in mouse with liver stagnation and spleen deficiency syndrome,and to explore the general characteristics of the model.Methods:After the induction of iminodipropionitrile (IDPN) by intraperitoneal injection,a rat model of multiple tic disorder with liver stagnation and spleen deficiency was established by eating dislocation,chronic restraint and excessive fatigue.The independent activity counts,stereotyped movement score,body weight,food intake,feces granu,thymus and spleen index,gastrin,D-xylose and other indexes were used to evaluate TS mouse of spleen deficiency.Results:In the model group and the liver stagnation and spleen deficiency group,the activity distance and the average speed of the rats in the same time were significantly increased compared with the blank control group.The food intake of rats in the liver stagnation and spleen deficiency group decreased,and the thymus index,spleen index,gastrin and D-xylose concentrations were statistically different (P<0.05).Conclusion:This model has relatively stable tic symptoms,and has some similar symptoms of liver stagnation and spleen deficiency syndrome,and to some extent has the surface validity of multiple tic disorder liver stagnation and spleen deficiency syndrome.