火针膝周密刺对膝关节骨性关节炎鼠关节形态及软骨LOXL2的影响

目的:探讨火针膝周密刺对膝关节骨性关节炎(Knee Osteoarthritis,KOA)大鼠行为学、关节形态及软骨中赖氨酰氧化酶相关蛋白2(LOXL2)影响性。方法:选择30只健康雄性大鼠,10只作为对照不作任何处理。20只大鼠建立KOA模型,成模后随机分成2组,分别予普通针刺和火针膝周密刺,1次/3 d,连续6次。观察各组大鼠Leauesne MG评分、关节软骨细胞情况,外周血及关节软骨转化生长因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)、基质金属蛋白酶3(MMP-3)、LOXL2水平变化。结果:治疗后火针组Leauesne MG评分中的局部疼痛刺激反应、步态、关节活动范围、关节肿胀度较对照组、模型组差异有统计学意义(P<0.05)。血清及关节软骨MMP-3、TGF-β1、TNF-α含量水平显著低于模型组(P<0.05),LOXL2显著高于模型组(P<0.05)。火针组电镜下软骨细胞形状规则、细胞核完整且饱满,微绒毛少量存在,内质网丰富,有散在的染色质。结论:火针膝周密刺能减轻K0A关节软骨损伤,降低炎性反应,改善软骨LOXL2水平,这可能是火针治疗KOA一个作用机制。

Effect of Around knee Fire Needling on Joint Morphology and LOXL2 in Cartilage of Rats with Knee Osteoarthritis

Objective:To investigate the effect of fire needle acupuncture around the knee on behavior,joint morphology and lysyl oxidase related protein 2(LOXL2)in cartilage of rats with knee osteoarthritis(Knee osteoarthritis,KOA).Methods:30 healthy male rats were selected and 10 rats were used as control without any treatment.KOA model was established in 20 rats.After the model was established,the rats were randomly divided into two groups.They were treated with common acupuncture and around knee fire needling respectively,once every 3 days for 6 consecutive times.The Leauesne MG score,articular chondrocytes and the levels of transforming growth factor-β1(TGF-β1),tumor necrosis factor-α(TNF-α),matrix metalloproteinase-3(MMP-3)and LOXL2 in peripheral blood and articular cartilage were observed.Results:After treatment,the local pain stimulation response,gait,joint range of motion and joint swelling of the Leauesne MG scoring in the fire needling group were significantly different from those in the control group and the model group(P<0.05).The levels of MMP-3,TGF-β1 and TNF-αin serum and articular cartilage in the fire needling group were significantly lower than those in the model group(P<0.05),while LOXL2 was significantly higher than that in the model group(P<0.05).Under electron microscope,the chondrocytes in the fire needling group had regular shape,complete and full nucleus,a small amount of microvilli,abundant endoplasmic reticulum and scattered chromatin.Conclusion:Fire needling around the knee can reduce the injury of K0A articular cartilage,reduce the inflammatory reaction and improve the level of cartilage LOXL2,which may be one of the mechanisms of fire needling in the treatment of KOA.