山银花、金银花微毒测试(Microtox)与安全性研究

目的:运用小鼠急性毒性试验和基于Microtox(微毒)技术的生物综合毒性评价技术对比研究金银花醇提物和山银花醇提物的毒性大小,探索Microtox技术应用于中药毒性快速评价的可行性。方法:小鼠一次性灌胃给予不同浓度的金银花醇提物、山银花醇提物,观察并记录小鼠的毒性反应及死亡情况,计算小鼠的半数致死量(LD50);利用Microtox技术测定金银花醇提物、山银花醇提物对发光菌发光强度抑制率,计算发光细菌半数抑制浓度(IC50),将2种毒性评价方法的结果进行比较。结果:小鼠灌胃金银花醇提物和山银花醇提物的半数致死量(LD50)值分别为81.29 g生药/kg、91.0 g生药/kg。采用生物综合毒性评价技术测得对发光细菌的半数抑制率分别为0.001 3 g生药/mL、0.002 2 g生药/mL。结论:应用小鼠急性毒性试验及基于Microtox技术的生物综合毒性评价测试金银花提取物和山银花提取物,2种评价结果具有一定的一致性,将Microtox技术用于金银花提取物和山银花提取物毒性的快速评价具有一定的可行性,但2种毒性评价方法的相关程度还有待扩大样本量进一步研究。

Study on Microtox and Safety of Honeysuckle and Lonicera

By using the acute toxicity test of mice and the comprehensive toxicity evaluation technology based on Microtox technology, to compare the toxicity of ethanol extracts from Honeysuckle and Lonicera Japonica, and to explore the feasibility of Microtox technology in rapid toxicity evaluation of traditional Chinese medicine. Methods:Mice were given ethanol extracts from Honeysuckle and Lonicera Japonica at different concentrations in a single gavage, and the toxicity reaction and death of the mice were observed and recorded, then calculated the median lethal dose(LD50)of the mice. The inhibition rate of ethanol extracts from Honeysuckle and Lonicera Japonica to the luminescence intensity of the luminescent bacteria was measured by Microtox technology, and calculated the half inhibitory concentration(IC50)of the luminescent bacteria. The results of the two toxicity evaluation methods were compared. Results:The median lethal dose(LD50)values of the ethanol extract from Honeysuckle and Lonicera Japonica were respectively crude drug 81.29 g/kg and crude drug 91.00 g/kg. The half-inhibition rate of luminescent bacteria measured by the bio-complex toxicity evaluation technique was crude drug 0.0013 g/mL and crude drug 0.0022 g/mL, respectively. Conclusion:The acute toxicity test in mice and the comprehensive toxicity test based on Microtox technology were used to test the extracts of Honeysuckle and Lonicera Japonica. The two kinds of evaluation results have a certain consistency. It is feasible to use Microtox technology to evaluate the toxicity of extracts from Honeysuckle and Lonicera Japonica, but the correlation between the two methods needs to be further studied with a larger sample size.