艾烟对SAMP8小鼠嗅球形态和嗅球内谷氨酸、γ 氨基丁酸和乙酰胆碱酯酶的影响〖JZ)〗〖HS)〗

目的：观察艾烟干预对快速老化SAMP8小鼠嗅球形态和嗅球内谷氨酸（Glu）、γ-氨基丁酸（GaBa）和乙酰胆碱酯酶（AChE）表达的影响，探讨艾烟的可能效应途径。方法：选取6月龄雄性快速老化模型SAMP8小鼠48只并随机分为艾烟组、模型组、嗅觉障碍组和嗅觉障碍艾烟组，以12只SAMR1作为空白对照组对照。3-甲基吲哚（3-MI）腹腔注射制作嗅觉障碍模型。艾烟组和嗅觉障碍艾烟组每日予以艾烟吸嗅30 min，其余组正常空气吸嗅。干预6周后通过苏木精-伊红（HE）染色观察各组嗅球形态，并利用酶联免疫吸附试验法比较嗅球内Glu、GaBa及AChE表达情况。结果：各组嗅球结构未见明显差异，艾烟组颗粒细胞密度略高于模型组、嗅觉障碍组和嗅障艾烟组。艾烟组Glu、AChE较模型组显著降低，差异有统计学意义（P<0.05），且AChE含量低于嗅觉障碍组，差异有统计学意义（P<0.05）。嗅障艾烟组Glu含量介于模型组和艾烟组之间，3组差异有统计学意义（P<0.05）。结论：一定浓度的艾烟干预可以良性调节嗅球内神经递质含量，并通过嗅觉通路的神经投射干预脑内神经递质的表达，改善氧化应激。

Effects of Moxa Smoke on Morphology of Olfactory Bulb and the Expression of Glu,Gaba and Ache in Olfactory Bulb of SAMP8 Mice

To observe the effects of moxa smoke on the olfactory bulb morphology and the expression of glutamate acid (Glu),gamma-aminobutyric acid (GaBa) and acetylcholinesterase (AChE) in the olfactory bulb of aging SAMP8 mice,in order to explore the possible pathway of moxa smoke effect.Methods:A total of 48 six-month-old male SAMP8 mice were randomly divided into 3 groups:a moxa smoke group,a model group,a dysosmia group and a dysosmia model treated with moxa smoke group.12 SAMR1 mice were used as control group.3-methylindole (3-MI) was injected intraperitoneally to produce an olfactory dysfunction model.The moxa smoke group and dysosmia model treated with moxa smoke group inhaled moxa smoke for 30 min every day.Other groups inhaled normal air.After 6 weeks'' intervention,the morphology of olfactory bulb was observed by HE staining,and the contents of Glu,GaBa and AChE in olfactory bulb were tested by ELISA.Results:There was no significant difference in olfactory bulb structure among the 5 groups.The density of granular cells in the inner plexus layer of the moxa smoke group was slightly higher than that of the model group,the dysosmia group and the dysosmia treated with moxa smoke group.Contents of Glu and AChE in moxa smoke group were significantly lower than those in model group (P<0.01,P<0.05),and its AChE content was lower than that in the dysosmia group (P<0.05).The content of Glu in dysosmia treated with moxa smoke group was between the model group and the moxa smoke group,and there was significant difference among the 3 groups (P<0.05).Conclusion:With certain concentration,moxa smoke intervention can benignly regulate the content of neurotransmitters in the olfactory bulb.Through the neural projection of the olfactory pathway,the expression of neurotransmitters in the brain can be interfered and the oxidative stress could be improved.