不同提取法炮制对水蛭体外溶栓活性的影响

目的:研究炮制对水蛭体外溶栓活性的影响。方法:采用水提取法和仿生提取法对水蛭净制品、烫制品、酒制品进行提取,选用纤维蛋白平板法和纤维蛋白原平板法,以平板上透明圈面积为指标进行纤溶活性测定;并建立体外血栓模型,通过血凝块的失重比进一步评价水蛭不同炮制品的溶栓活性。结果:水蛭在炮制前后均能在纤维蛋白平板上产生透明圈。采用水提取法时,烫制品和酒制品的溶圈面积减小,炮制后纤溶活性降低,活性顺序为:净制品酒制品烫制品。而采用仿生提取法时,显示烫制和酒制后水蛭纤溶活性升高,活性顺序为:烫制品酒制品净制品。体外血栓模型中,血凝块失重比结果与纤溶活性测定结果一致,采取水提取法时,炮制使溶栓活性降低,而采用仿生提取法时显示炮制使溶栓活性增加。结论:与水提取法比较,仿生提取法更符合水蛭经口服后在人体内的消化吸收过程,其结果更有说服力。传统炮制可增强水蛭溶栓活性。

Effects of Water Extraction Method and Bionic Extraction Method on Antithrombotic Activity of Processed Products of Whitmania Pigra

To explore the effects of different processing methods on the antithrombotic activity of Whitmania Pigra by different extraction methods.Methods:The water extraction method and the bionic extraction method were used respectively to extract the antithrombosis active components in W.pigra hanging dried products, talcum powder fried products and wine products.In the case of the transparent circle area of the fibrin plate method and the fibrinogen plate method were used as the activity indexes to evaluate the fibrinolytic activities of different processed W.pigra.Then, for the purpose of further determining the antithrombosis activity, the thrombus model in vitro was established in order to calculate further the dissolution rate of blood clot.Results:W.pigra could produce transparent rings on the fibrin plate before and after processing.In the sample of the water extraction method, it was found there existed not only decreased the area of transparent rings but also weakened activity in fibronolysis after processing both in the talcum powder fried products and the wine products, and the activity order was as follows:hanging dried products>wine products>talcum powder fried products, the results of whom were in agreement with the results of the weight loss ratio of the dissolved blood clot in vitro.In the sample of the bionic extraction method, all the results indicated the fibrinolytic activity and the thrombolysis rate of W.pigra after wine product and talcum powder fried product were increased.Conclusion:When compared with the water extraction method, the bionic extraction method was more similar to the absorption process of W.pigra in human digestive system after oral administration, and the extracted ingredients of the bionic extraction method were more consistent with human digestion and absorption.Therefore, the traditional processing method can enhance the antithrombosis activity of W.pigra.