芪术抗癌方对肝癌HepG2细胞增殖和凋亡的影响

目的:探讨芪术抗癌方对肝癌HepG2细胞增殖与凋亡的调控作用。方法:体外培养HepG2细胞,加入不同浓度芪术抗癌方(0,250,500,1000 mg/L)处理48 h。采用细胞计数试剂盒8(CCK-8)检测细胞活力,采用免疫印记法检测凋亡相关蛋白c-Caspase-3和c-Caspase-9表达变化。结果:CCK-8结果显示芪术抗癌方可呈浓度依赖性抑制HepG2细胞增殖,与对照组比较,差异有统计学意义(P<0.05),1000 mg/L组抑制效果最好,芪术抗癌方刺激HepG2细胞后,凋亡相关蛋白c-Caspase3及c-Caspase9表达增加,呈浓度依赖性,在1000 mg/L组达到最高且差异有统计学意义(c-Caspase3增加1.83倍,P<0.05;c-Caspase9增加1.06倍,P<0.05)。结论:芪术抗癌方可抑制肝癌HepG2细胞增殖并诱导细胞凋亡。

Effects of Qizhu Kangai Formula on Proliferation and Apoptosis of HepG2 Heptoma Cells of Liver Cancer

To study the effects of Qizhu Kangai Formula on the proliferation and apoptosis of HepG2 heptoma cells of liver cancer.Methods:The HepG2 cells were cultured in vitro and treated with different concentration of Qizhu Kangai Formula (0,250,500,1 000 mg/L ) for 48 h.The living cells were detected by cell counting kit 8 ( CCK8 ) and the protein expression of c-caspase3 and c-caspase9 were observed by Western-blot.Results:CCK8 showed that Qizhu Kangai Formula could inhibit HepG2 cells proliferation in concentration-dependent manner and had significant differences with the control group (P<0.05).Among them,the 1000 mg/L group had the best inhibition effects.Further studies found that the expression of the apoptotic proteins c-caspase3 and c-caspase9 increased after the stimulating HepG2 cells by Qizhu Kangai Formula and was concentration-dependent and was highest in the 1000 mg/L group with statistical significance ( c-caspase3 increased 1.83 times,P<0.05; c-caspase9 increases by 1.06 times,P<0.05 ).Conclusion:Qizhu Kangai Formula could inhibit the proliferation and induce apoptosis of HepG2 heptoma cells.