灵芝多糖对急性酒精性肝损伤小鼠肝脏脂肪沉积及N LRP3炎性小体表达的影响

目的:探讨灵芝多糖对急性肝损伤小鼠肝脏脂肪沉积及NLRP3炎性小体表达的影响。方法:将48只健康KM小鼠随机分为对照组、模型组及实验组,每组16只。模型组及实验组小鼠以慢性酒精喂养联合急性酒精灌胃法构建急性酒精性肝损伤小鼠模型,且实验组建模期间灌胃150 mg/kg灵芝多糖,1次/d。以全自动生化分析仪检测小鼠肝功能及脂代谢指标;酶联免疫吸附试验(ELISA)法检测血清炎性反应递质水平;苏木精-伊红(HE)染色检测小鼠肝组织病理学形态;油红O染色观察肝脏组织脂肪沉积;蛋白免疫印迹法(WB)检测肝组织NLRP3炎性小体表达。结果:各组小鼠的血清谷丙转氨酶(ALT),血清谷草转氨酶(AST),血清白细胞介素-1β(IL-1β),血清白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α),油红O染色评分,肝组织NLRP3炎性小体相对表达量等,模型组与对照组比较,血清AST、ALT、三酰甘油、总胆固醇、游离脂肪酸、IL-1β、IL-6、TNF-α水平,油红O染色评分及肝组织NLRP3炎性小体相对表达量升高,而实验组均较模型组降低,差异有统计学意义(P<0.05)。结论:灵芝多糖可通过抑制肝脏组织NLRP3炎性小体表达,抑制急性酒精性肝损伤小鼠炎性反应及肝脏脂肪沉积,有效缓解肝组织损伤,恢复其肝功能。

Effects of Ganoderma Polysaccharide on Liver Fat Deposits and NLRP3 Inflammatory Corpuscle Expression of Mice with Acute Liver Injury

Objective:To explore the effects of ganoderma polysaccharide on liver fat deposits and NLRP3 inflammatory corpuscle expression of mice with acute liver injury.Methods:A total of 48 healthy KM mice were randomly divided into a control group,a model group and a test group,with 16 cases were in each group.Mice in the model group and the test group were constructed an acute alcoholic liver injury mice model combined the chronic alcohol feeding with the acute alcohol lavage method,and mice in the test group were treated by the lavage method of feeding 150 mg/kg-1 ganoderma polysaccharide,once a day.The liver function and the lipid metabolism indexes of mice were detected by an automatic biochemical analyzer;the serum inflammatory factor contents were detected by an enzyme-linked immunosorbent assay(ELISA)method;the liver tissue pathology morphology of mice was observed by a hematoxylin and eosin(HE)staining;the liver tissue fat deposits of mice were observed by an oil red O staining;the NLRP3 inflammatory corpuscle expression of mice was detected by an western blot(WB)method.Results:The experimental results showed the relevant expressions of mice in every group including the serum alanine aminotransferase(ALT),serum glutamic-oxaloacetic transaminase(AST),serum interleukin-1β(IL-1β),the serum interleukin-6(IL-6),and the tumor necrosis factor-α(TNF-α),as well as the oil red O staining scores and the liver tissue NLRP3 inflammatory corpuscle were the same.Comparing the model group with the control group,it could be seen that the relevant expressions of the serum AST,ALT,triacylglycerol,total cholesterol,free fatty acids,IL-1β,IL-6,TNF-α,and the oil red O staining scores,and the liver tissue NLRP3 inflammatory corpuscle in the model group increased;compared with the model group,the test group level was lower,and the difference was statistically significant(all P<0.05).Conclusion:Ganoderma polysaccharide can inhibit liver tissue NLRP3 inflammatory corpuscle protein expression,and the inflammatory response and liver fat deposits of acute alcoholic liver injury mice,so as to relieve the liver tissue damage and to restore the liver function effectively.