加味四君子对宫颈癌大鼠模型癌组织中Caspase-3、Caspase-7、Survivin表达的干预作用

目的:观察加味四君子汤对宫颈癌模型癌组织Caspase-3、Caspase-7、Survivin表达的影响,以探明加味四君子汤治疗宫颈癌的机制。方法:将30只Wistar雌性大鼠随机分为空白组、模型组及干预组,其中空白组10只,模型组及干预组各9只。其中空白组大鼠不接受造模及干预,模型组大鼠仅接受模型制备,干预组大鼠接受模型制备及加味四君子汤灌胃,连续治疗8周,疗程结束后比较各组宫颈癌病灶大小,同时用免疫组化法检测各组宫颈癌组织Caspase-3、Caspase-7、Survivin的水平表达变化。结果:1)空白组未出现宫颈癌病灶,模型组病灶体积(1 573. 48±11. 24) mm3,干预组病灶体积(683. 23±4. 81) mm3,各组宫颈癌病灶大小差异有统计学意义(P 0. 05),干预组明显较模型组小。2) 2组接受造模术大鼠Caspase-3、Caspase-7浓度均有不同程度下调,Survivin表达升高,其中干预组大鼠经过为期8周的干预后Caspase-3、Caspase-7浓度上调,Survivin浓度下降,干预组改善明显优于模型组,差异有统计学意义(P 0. 05)。3)经过相关分析显示,癌灶体积与Caspase-3、Caspase-7呈负相关关系,与Survivin有正相关关系。结论:加味四君子可明显缩小宫颈癌病灶体积,其作用机制可能与调节Caspase-3、Caspase-7、Survivin表达有关。

Intervention Effects of the Modified Sijunzi Decoction on the Expression of Caspase-3,Caspase-7,Survivin in the Cervical Cancer Tissue

To observe the impact of Modified Sijunzi Decoction on the expression of Caspase-3,Caspase-7,Survivin in the cervical cancer model tissue,and to explore the mechanism of modified Sijunzi Decoction in the treatment of cerical cancer.Methods:A total of 30 Wistar female rats were randomly divided into blank group,model group and intervention group,10 in each group.The blank group rats was not modeled and intervened,and the model group only accepted model preparation,the intervention group accepted model preparation and modified Sijunzi Decoction lavage,which continued for 8 weeks.A comparison was made of the cervical cancer lesion size of these groups after treatment,at the same time the immunohistochemical method was used to detect the expression level changes of Caspase-3,Caspase-7 and Survivin in each cervical cancer tissue.Results:1) The blank group did not have cervical lesion,the cervical lesion volume of the model group were (1573.48±11.24) mm3,the cervical lesion volume of the intervention group were (683.23±4.81) mm3.The difference in cervical lesion size had statistical significance (P<0.05),and the intervention group was significantly smaller compared with the model group. 2) The concentration of Caspase-3 and Caspase-7 in 2 groups was lower in different extent,and the expression of Survivin increased.After 8 weeks of intervention,the concentration of Caspase 3 and Caspase-7 in the intervention group increased and the concentration of Survivin decreased.The improvement of the intervention group was obviously better than that of the model group,and the difference was statistically significant (P<0.05). 3) The correlation analysis showed that cancer volume were negatively related to Caspase-3 and Caspase-7,but with positive correlation to Survivin.Conclusion:Modified Sijunzi Decoction can obviously reduce cervical lesion volume,and its mechanism may be related to regulation of the expression of Caspase-3,Caspase-7 and Survivin.