香鳞毛蕨95%乙醇提取物的急性毒性研究

目的:探讨香鳞毛蕨95%乙醇提取物对小鼠的急性毒性。方法:采用健康昆明种小鼠,灌胃给予香鳞毛蕨95%乙醇提取物,给药剂量范围为10.00 g/kg~24.00 g/kg,观察小鼠中毒症状并记录死亡动物情况,采用Bliss法测定半数致死量(LD50,Median Lethal Dose);灌胃给予香鳞毛蕨95%乙醇提取物,给药剂量分别为32 g/kg、16 g/kg、8 g/kg,对各组小鼠进行生化检查和病理组织形态学检查。结果:香鳞毛蕨95%乙醇提取物的小鼠LD50为13.53 g/kg;病变部位为心肌凝固性坏死,肾皮质、髓质内可见肾小管变性坏死脱落,肝细胞轻度浊肿,空泡变性,脾脏及肺脏等未见明显病变。结论:香鳞毛蕨95%乙醇提取物具有一定的毒性。

Acute Toxicity of 95% Ethanol Extracts from Dryopteris fragrans (L.) Schott

To study the acute toxicity of 95% ethanol extracts from Dryopteris fragrans (L.) Schott in mice. Methods:The Dryopteris fragrans 95% ethanol extracts were given to the healthy Kunming mice by intragastric administration. The dose range of 95% ethanol extracts were 10.00 g/kg~24.00 g/kg. The movement and toxic symptoms were observed and the number of dead mice were recorded. LD50 were calculated by Bliss'' method. Pathological changes were examined by pathological section. Results:LD50 of the 95% ethanol extracts were 13.53 g/kg, respectively. Pathological section indicated that the myocardial coagulation necrosis, renal tubular degeneration and necrosis, and mild swelling of liver cells, vacuole degeneration, while other organs such as the spleen and lung were with no significant pathological changes. Conclusion:The 95% ethanol extracts of Dryopteris fragrans possesses certain toxicity.