葛花枳椇子配伍使用对醉酒小鼠体内乙醇代谢过程的影响

目的:通过检测急性乙醇中毒小鼠体内乙醇浓度、乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)的在不同时间的变化水平,探讨葛花枳蓂子配伍应用是否优于单独使用。方法:将ICR小鼠分为空白组、模型组、阳性药组、单用葛花、枳蓂子组及配伍1、2、3组。选取预防给药的方法,即给药30min后灌酒复制模型,分别于酒后05h,1h,2h,3h摘眼球取血及肝脏组织。酶法测定酒后不同时间点小鼠血中乙醇浓度、肝中ADH和ALDH水平。结果:醉酒小鼠血中乙醇浓度在2h达到峰值,葛花、枳蓂子配伍1、2、3组小鼠体内乙醇浓度均较模型组显著降低(P0.05);小鼠肝脏中ADH水平在1h达峰,配伍2、3组可显著升高小鼠酒后1h肝脏中ADH的水平(P0.05),在2h、3h后,只有配伍3组效用较为明显(P0.05);小鼠肝脏中ALDH水平在2h到达峰值,配伍3组可显著增加其水平,其余各组有增加的趋势但差异无统计学意义。结论:葛花、枳蓂子配伍使用效果优于单独应用,可降低酒后不同时间点小鼠血中乙醇浓度,其机制可能与激活肝脏中ADH与ALDH活性有关,从而起到预防醉酒的作用。

Effectiveness of Compatibility of Flos Puerariae and Semen Hoveniae on metabolic process of alcohol for acute alcoholism mice

To discuss whether using the compatibility of Flos Puerariae and Semen Hoveniae or use them respectively was better through testing the changes of alcohol concentration, alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH) in acute alcoholism mice at different time points. Methods:ICR mice were randomly divided into control group, model group, positive group, Flos Puerariae(FP) group, Semen Hoveniae(SH) group, and combination group 1,2,3. Preventive administration method was taken, that was, feeding the mice alcohol to establish the model after 30 min administration. The blood and liver tissue were collected after 0.5 h,1 h, 2 h and 3 h respectively.The concentration of ethanol and the activity of ADH and ALDH in liver at different time point were measured by biochemical enzyme method. Results:The concentration of ethanol in blood reached the peak 2 h after intragastric administration. Compared with the model group, concentration of ethanol in blood in combination group 1,2,3 significantly decreased after 2 h (P<0.05); the level of ADH in the liver of mice reached peak 1 h later, and combination group 2,3 can significantly enhance liver ADH activity 1 h later for the drunken mice (P<0.05). Only combination group 3 was obvious effective in 2 h, 3 h; the level of ALDH in the liver of mice reached a peak at 2 h, and the combination group 3 had a significant increase. As for other groups, they tend to increase but the difference was not statistically significant.Conclusion:The effect of compatibility of Flos Puerariae and Semen Hoveniae was better than single application, it can decrease the concentration of alcohol in the blood of mice. Activating the activity of ADH and ALDH in the liver, inhibit the absorption of ethanol in the digestive tract may play a preventive role.