| 栀子叶片愈伤组织诱导优化研究 |
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| 引用本文： | 田亚,徐志超,胡开治,王华磊,宋经元.栀子叶片愈伤组织诱导优化研究[J].世界中医药,2020,15(9):1275-1278. |
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| 作者姓名： | 田亚 徐志超 胡开治 王华磊 宋经元 |
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| 作者单位： | 贵州大学农学院药用植物繁殖与栽培重点实验室,贵阳,550025;中国医学科学院/北京协和医学院药用植物研究所,国家中医药管理局中药资源保护重点研究室,中药资源教育部工程研究中心,北京,100193;中国医学科学院/北京协和医学院药用植物研究所,国家中医药管理局中药资源保护重点研究室,中药资源教育部工程研究中心,北京,100193;重庆市药物种植研究所,重庆,408435;贵州大学农学院药用植物繁殖与栽培重点实验室,贵阳,550025 |
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| 基金项目： | 国家自然科学基金项目（81973424）；重庆市社会事业与民生保障科技创新专项（cstc2017shmsA130021） |
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| 摘 要： | 目的:对栀子(Gardenia jasminoides Ellis)叶片愈伤组织诱导进行优化研究。方法:通过以叶片为外植体,MS和WPM为基本培养基,添加不同激素组合(6-BA、NAA和IBA)进行愈伤组织诱导研究。结果:栀子叶片在WPM+6-BA 0.5 mg/L+NAA 0.5 mg/L以及6-BA 1.0 mg/L+NAA 0.1(或0.5)mg/L中均有100%的诱导率;在MS培养基上,MS+6-BA 1.0(或1.5)mg/L+NAA 0.5 mg/L的诱导率最好,分别为70%和74%。栀子叶片愈伤组织在再分化培养基上均未诱导出丛生芽。结论:WPM培养基比MS培养基更易诱导栀子叶片愈伤组织,且栀子愈伤组织的再分化较困难。
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| 关 键 词： | 栀子 叶片 愈伤组织诱导 MS培养基 WPM培养基 6-BA NAA IBA |
| 收稿时间： | 2020/2/10 0:00:00 |
Optimization of Callus Induction in Leaves of Gardenia jasminoides |
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| TIAN Y,XU Zhichao,HU Kaizhi,WANG Hualei,SONG Jingyuan.Optimization of Callus Induction in Leaves of Gardenia jasminoides[J].World Chinese Medicine,2020,15(9):1275-1278. |
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| Authors: | TIAN Y XU Zhichao HU Kaizhi WANG Hualei SONG Jingyuan |
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| Institution: | 1 Key Laboratory of Propagation and Cultivation on Medicinal Plants,College of Agriculture,Guizhou University,Guizhou 550025,China; 2 Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Key Lab of Chinese Medicine Resources Conservation,National Administration of Traditional Chinese Medicine of the People''s Republic of China,Engineering Research Center of Chinese Medicine Resource,Ministry of Education,Beijing 100193,China; 3 Chongqing Institute of Medicinal Plant Cultivation,Chongqing 408435,China |
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| Abstract: | To study the optimization of leaf callus induction in Gardenia jasminoides Ellis.Methods:The callus induction was studied using leaves as explant,MS and WPM as basic medium adding different hormone combinations(6-BA,NAA and IBA).Results:Gardenia leaves had a 100% of callus induction rate in WPM+6-BA 0.5 mg/L+NAA 0.5 mg/L or 6-BA1.0 mg/L+NAA 0.1(or 0.5)mg/L,while 70% and 74% were the best induction rates in MS+6-BA 1.0(or 1.5)mg/L+NAA 0.5 mg/L,respectively.The leaf callus of Gardenia did not induce cluster buds in the redifferentiation medium.Conclusion:Compared with MS medium,the callus was easy to be induced in WPM medium,and the redifferentiation of Gardenia callus was difficult. |
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| Keywords: | Gardenia jasminoides Ellis leaves Callus induction MS medium WPM medium 6-BA NAA IBA |
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