雷公藤配伍甘草减毒大鼠体内尿液中的小分子代谢物以及相应生物代谢通路

目的:分析雷公藤配伍甘草减毒大鼠体内尿液中的小分子代谢物以及相应生物代谢通路。方法:选取30只SD大鼠正常饲养5 d,随机分为3组,即空白对照组、雷公藤组、雷公藤配伍甘草组,每组10只。应用HPLC-MS/MS-IT-TOF检测各组大鼠的尿液样本,并且按照偏最小二乘法-判别分析(PLS-DA)多元统计学方法以筛选并鉴定潜在的生物标志物;在此基础上推测其可能影响到的生物代谢通路。结果:大鼠尿液代谢组学分析结果显示,雷公藤配伍甘草组、雷公藤组和空百对照组分别聚集在不同的象限区域内;共鉴定出14个潜在的生物标志物,并且推测出嘌呤代谢和硒氨基酸代谢最具显著影响。结论:雷公藤配伍甘草减毒的机制复杂,其中可能通过调控嘌呤代谢和硒氨基酸代谢通路而实现。

Analysis of Toxicity Reduction on Tripterygium Wilfordii Combined with Radix Glycyrrhizae by Using Metabolomics of Rat Urine

To analyze the changes of small molecular metabolites in urine of rats administrated by tripterygium wilfordii combined with Radix Glycyrrhizae,and the involved metabolic pathways by metabolomics.Methods:On the basis of the previous experiment,the rats were given different experimental intervention,specifically divided into tripterygium wilfordii group,tripterygium wilfordii combined with Radix Glycyrrhizae group and blank control group; HPLC-MS/MS-IT-TOF was used to detect urine samples of rats in each group,and PLS-DA multivariate statistical method was used to screen and identify potential biomarkers.On this basis,it is speculated that it may affect the biological metabolic pathways.Results:The results of urine metabolomics analysis showed that the groups were clustered in different quadrants.We together identified 14 potential biomarkers and speculated that purine metabolism and selenoamino acid metabolism had the most significant effects.Conclusion:The mechanism of tripterygium wilfordii combined with Radix Glycyrrhizae is complex,which may be achieved by regulating purine metabolism and selenoamino acid metabolism pathway.