P2X2受体在肝郁脾虚证小鼠模型前额皮质中的表达

目的:研究胞外ATP特异性P2X2受体的表达与肝郁脾虚证发生之间的关系,为深入了解胞外ATP的释放现象与肝郁脾虚证的发生机制之间的关系奠定基础。方法:48只C57BL/6J小鼠随机分为正常组、模型组、逍遥散组和氟西汀组,除正常组外其余各组小鼠均接受复合应激21 d,建立肝郁脾虚证小鼠模型。采用免疫组化、western blot和实时荧光定量PCR方法检测各组小鼠前额皮质P2X2受体蛋白和基因的表达。结果:与正常组比较,模型组小鼠前额皮质P2X2受体蛋白表达显著下降(P0.05),而P2X2受体mRNA表达差异无统计学意义,但存在一定的下降趋势(P0.05);与模型组比较,逍遥散组和氟西汀组小鼠前额皮质P2X2受体蛋白表达显著升高(P0.05),氟西汀组小鼠前额皮质P2X2受体mRNA表达显著升高(P0.05)。结论:本研究结果表明基于复合应激的肝郁脾虚证小鼠前额皮质P2X2受体的表达存在减少的趋势,其可能是诱导肝郁脾虚证发生的一个重要原因。

The expression of P2X2 receptor in prefrontal cortex of mice with syndrome of liver depression and spleen deficiency

To study the relationship between the expression of specific P2X2 receptor of ATP and occurrence of syndrome of liver depression and spleen deficiency, in order to lay the foundation for the study on relationship between the release phenomenon of ATP and occurrence of syndrome of liver depression and spleen deficiency. Methods:A total of 48 C57BL/6J mice were randomly divided into 4 groups as normal group, model group, Xiaoyaosan group, and Fluoxetine group. All the mice, except the normal group, received multiple stresses for 21 days to establish the mice model of syndrome of liver depression and spleen deficiency. Immunohistochemical technique, Western blot, and qPCR were used to test the expression of protein and mRNA of P2X2 receptor in prefrontal cortex of mice. Results:Compared with the normal group, the expression of protein of P2X2 receptor of mice in model group decreased significantly (P<0.05), and the expression of mRNA of P2X2 receptor of mice in model group declined, although it was not significant (P>0.05). Compared with model group, the expression of protein of P2X2 receptor of mice in Xiaoyaosan and Fluoxetine groups increased significantly (P<0.05), and the expression of mRNA of P2X2 receptor of mice in Fluoxetine group also increased (P<0.05). Conclusion:Induced by multiple stresses, the expression of P2X2 receptor in prefrontal cortex of mice with syndrome of liver depression and spleen deficiency tends to be reduced, which might be an important reason of occurrence of syndrome of liver depression and spleen deficiency.