3种不同基质对黄连素软膏体外透皮吸收的影响

目的:为提高黄连素软膏中有效成分黄连素的透皮吸收的效果,通过测定3种不同基质(油脂型基质、乳剂基质、水溶性基质)制成的黄连素软膏中黄连素的体外透皮吸收情况,寻找适合于外用黄连素的软膏剂类型。方法:制备3种不同基质的黄连素软膏,以小鼠离体去毛皮肤为渗透膜,使用智能透皮试验仪进行体外透皮扩散实验,在相同条件下于各规定时间吸取吸收液,以分光光度法在344 nm处测量黄连素(以盐酸小檗碱计)的吸收度,并用回归方程计算黄连素的透皮吸收量。结果:3种基质的黄连素软膏在12 h内均有一定吸收,其中乳剂型黄连素软膏的吸收率最高,其次是水溶性,油脂型最差。结论:中药外用膏剂的基质类别对药物的透皮吸收有明显的影响,本实验中由于黄连素的疏水性,最适合其透皮吸收的软膏的基质类型为乳剂型。

The In Vitro Percutaneous Absorption of Berberine Hydrochloride Ointments with Three Dissolvents

To compare the in vitro percutaneous absorption of the berberine hydrochloride ointments with oil, emulsion and water-soluble dissolvents. Methods:The above three kinds of ointments were applied to the equal areas of the in vitro dehaired epidermis of mice. Under the same condition, the absorbed liquid degree by epidermis was collected within various durations. The absorbance of liquid was measured with spectrophotometry at 344 nm. Results:Within 12 hours, all of the three ointments were absorbed by epidermis to a certain degree, where the percutaneous absorbance of emulsion ointment was the highest, followed by water-soluble ointment, and the oil-like ointment which had the lowest absorbance. Conclusion:Dissolvent type of TCM ointment has obvious influence on the percutaneous absorption of the drug.