酸枣仁汤对抑郁症模型大鼠海马和皮层脑源性神经营养因子及酪氨酸激酶B基因表达的影响

目的：探讨酸枣仁汤对慢性应激抑郁模型大鼠海马和皮层脑源性神经营养因子（BDNF）、酪氨酸激酶B（TrKB）基因表达的影响。方法通过孤养与慢性刺激相结合制备抑郁症模型大鼠，灌胃给予酸枣仁汤进行干预，采用RT-PCR法检测大海鼠马、皮层中BDNF和TrKB的mRNA表达。结果酸枣仁汤高、中剂量组，氯丙咪嗪组皮层部位BDNF、TrKB mRNA表达量（分别为0.160±0.028、0.200±0.024、0.560±0.032、0.399±0.041、0.124±0.016、1.224±0.015）均高于模型组（分别为0.032±0.008、0.001±0.000），差异有统计学意义（P＜0.01）。酸枣仁汤高、中剂量组、氯丙咪嗪组海马部位BDNF、TrKB mRNA表达量（分别为0.213±0.094、0.639±0.023、1.032±0.015、1.089±0.014、1.580±0.012、1.860±0.019）均高于模型组（分别为0.021±0.015、0.125±0.013），差异有统计学意义（P＜0.01）。酸枣仁汤低剂量组皮层和海马部位BDNF、TrKB mRNA表达与模型组比较，差异无统计学意义（P＞0.05）。结论酸枣仁汤可上调BDNF和TrKB基因表达，具有抗抑郁作用。

The Effects of Suanzaoren decoction on hippocampus,cortex BDNF and TrKB geneexpression in depression rats

Objective To investigate the effect of Suanzaoren Decoction on hippocampus, cortex BDNF and TrKB gene expression in depression model rats. Methods Depression rat models were established by social-isolated raise and chronic stress stimulation. Suanzaoren decoction was administrated to the models. RT-PCR was adopted to detect the expression of mRNA BDNF and TrKB genes. Results The mRNA expression of BDNF and TrKB in cortex of Suanzaoren decoction high dose group、medium dose group and clomipramine group（0.213±0.094, 0.639±0.023, 1.032±0.015, 1.089±0.014, 1.580±0.012, 1.860±0.019）were all higher than the model group（0.032±0.008, 0.001±0.000）, showing a significant difference among four groups （P〈0.01）, and the mRNA expression of BDNF and TrKB in hippocampus of Suanzaoren decoction high dose group, medium dose group and clomipramine group（0.213±0.094, 0.639±0.023, 1.032±0.015, 1.089±0.014, 1.580± 0.012, 1.860±0.019）were higher than the model group （0.021±0.015, 0.125±0.013）, there was a significant difference between four groups（P〈0.01）. Compared with the model group, the mRNA expression of low-dose group of Suanzaoren decoction in both cortex and hippocampus of BDNF and TrKB was not significantly different to the model group（P＆gt;0.05）. Conclusion Suanzaoren decoction can increase the expression of BDNF and TrKB gene, promote neuronal proliferation, and resist depression.