子宫内膜样腺癌组织中内分泌细胞及其雌、孕激素受体的研究

目的　探讨子宫内膜样腺癌组织中内分泌细胞与雌、孕激素受体的关系。方法　采用免疫组化S -P法 ,检测 5 0例子宫内膜样腺癌组织中嗜铬素A(CgA )、雌激素受体 (ER )及孕激素受体 (PR )的表达情况 ,并对CgA阳性的子宫内膜样腺癌组织分别进行CgA /ER及CgA/PR双重免疫组化染色。 结果　子宫内膜样腺癌组织中CgA阳性率为 44 .0 % (2 2 /5 0 )。ER阳性率为2 8.0 % (14 /5 0 ) ,PR阳性率为 5 0 .0 % (2 5 /5 0 )。子宫内膜样腺癌CgA阳性组与阴性组之间ER表达率无显著性差异 (P >0 .0 5 ) ,但PR表达率有显著性差异 (P <0 .0 5 )。 2 2例CgA阳性子宫内膜癌细胞中CgA /ER同时表达率为 10 .1% (2 2 1/2 2 0 8个细胞 ) ,CgA/PR同时表达率为 2 0 .1% (4 90 /2 44 0个细胞 )。结论　子宫内膜癌组织出现较多的内分泌细胞及ER、PR的缺失是肿瘤异质性的表现。内分泌细胞不仅影响肿瘤细胞的生长 ,而且可能与子宫内膜癌对激素治疗的抗性或激素治疗后的复发有关

Study on Endocrine Cells with their Estrogen Receptors and Progesterone Receptors in Endometrioid Adenocarcinoma

Objective To study the relationship between endocrine cells(EC) and estrogen receptor(ER) and progesterone receptors(PR) in endometrioid adenocarcinoma.Methods Endocrine cells were identified by immunohistochemical staining of Chromogranin A in 50 cases of endometrioid adenocarcinoma.The ER and PR were also stained with immunohistochemistry by its monoclone antibody.ER and PR status of EC were observed by CgA/ER and CgA/PR double staining.Results The positive rate of CgA in endometrioid adenocarcinoma was 44.0%(22/50).The positive rate of ER and PR in endometrioid adenocarcinoma was 28.0%(14/50) and 50.0%(24/50),respectively.There was a significant difference( P <0.05) in PR positive rate between the groups of CgA positive and negative.The double staining showed that co expressing rate of CgA/ER and CgA/PR was 10.1%(221/2 208) and 20.1%(490/2 440),respectively.Conclusion The presence of endocrine cells and the absence of ER and PR in endometrioid adenocarcinoma shows heterogeneity of tumors.Endocrine cells do not only relate to the growth of tumor,but also may be related to hormone resistant or relapse after hormonal treatment.