| 应用PFGE分析广州稻叶型霍乱弧菌的同源性 |
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| 引用本文： | 李孝权,庞杏林,张欣强,邓志爱,李美霞,陈守义,邓小玲,王鸣. 应用PFGE分析广州稻叶型霍乱弧菌的同源性[J]. 热带医学杂志, 2011, 11(4): 428-432 |
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| 作者姓名： | 李孝权 庞杏林 张欣强 邓志爱 李美霞 陈守义 邓小玲 王鸣 |
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| 作者单位： | 1. 广州市疾病预防控制中心,广东,广州,510080
2. 广东省疾病预防控制中心,广东,广州,510600 |
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| 基金项目： | 广州市医药卫生科技项目，广东省医学科研基金 |
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| 摘 要： | 目的 分析广州40株稻叶型霍乱弧菌的毒力基因和PFGE特征,探讨菌株的同源性和广州稻叶型霍乱弧菌流行的特点.方法 应用PCR方法 检测稻叶型菌株的4种毒力基因,采用限制性内切酶Not I对菌株进行PFGE分型,使用BioNumerics Version 4.0软件(复选Dice相关系数和UPGMA方法 )对菌株进行聚类...
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| 关 键 词： | 霍乱弧菌 毒力基因 脉冲场凝胶电泳分析(PFGE) 分子分型 同源性 |
Homology analysis of strains of Vibrio cholerae serotype Inaba isolated in Guangzhou by pulsed-field gel electrophoresis analysis(PFGE) |
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| LI Xiao-quan,PANG Xing-lin,ZHANG Xin-qiang,DENG Zhi-ai,LI Mei-xia,CHEN Shou-yi,DENG Xiao-ling,WANG Ming. Homology analysis of strains of Vibrio cholerae serotype Inaba isolated in Guangzhou by pulsed-field gel electrophoresis analysis(PFGE)[J]. Journal Of Tropical Medicine, 2011, 11(4): 428-432 |
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| Authors: | LI Xiao-quan PANG Xing-lin ZHANG Xin-qiang DENG Zhi-ai LI Mei-xia CHEN Shou-yi DENG Xiao-ling WANG Ming |
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| Affiliation: | LI Xiao-quan1,PANG Xing-lin1,ZHANG Xin-qiang1,DENG Zhi-ai1,LI Mei-xia1,CHEN Shou-yi1,DENG Xiao-ling2,WANG Ming1 (1.Guangzhou Centers for Disease Control and Prevention,Guangdong,Guangzhou 510080,2.Guangdong Centers for Disease Control and Prevention,Guangzhou 510600,China) |
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| Abstract: | Objective To analyze the characteristics of pathogenicity-associated genes and PFGE features of 40 strains of Vibrio cholerae(V.cholerae)serotype Inaba isolated in Guangzhou,and study homology and features of cholera epidemic caused by V.cholerae.Methods Polymerase Chain Reaction(PCR)assay was utilized to detect the four pathogenicity-associated genes of these isolates.PFGE using restriction enzyme Not I was employed in the molecular subtyping of V.cholerae isolates and PFGE patterns were analyzed by BioNum... |
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| Keywords: | Vibrio cholerae pathogenicity-associated gene Pulsed-Field Gel Electrophoresis analysis (PFGE) molecular subtyping homology |
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