| 重组人源细胞珠蛋白单抗制备及检测方法建立 |
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| 引用本文： | 汤金萍,魏威,王萍,李珍,李莹,董文其. 重组人源细胞珠蛋白单抗制备及检测方法建立[J]. 热带医学杂志, 2011, 11(4): 375-378,400,387,391 |
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| 作者姓名： | 汤金萍 魏威 王萍 李珍 李莹 董文其 |
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| 作者单位： | 南方医科大学生物技术学院生物制药系,广东,广州,510515 |
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| 基金项目： | 广东省科技重点资助项目 |
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| 摘 要： | 目的 制备重组人源细胞珠蛋白(recombinant human Cytoglobin,rhCygb)单克隆抗体,并建立检测该蛋白双抗体夹心ELISA法,为下一步研究rhCygb药代动力学做准备.方法 用纯化的rhCygb免疫BALB/c小鼠,采用甲基纤维素半固体培养基法获得抗rhCygb的单克隆抗体杂交瘤细胞,间接E...
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| 关 键 词： | 重组人源细胞珠蛋白 单克隆抗体 ELISA双抗体夹心法 定量检测方法 |
Preparation of monoclonal antibodies against recombinant human cytoglobin and establishment of method for detection of the protein |
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| TANG Jin-ping,WEI Wei,WANG Ping,LI Zhen,LI Ying,DONG Wen-qi. Preparation of monoclonal antibodies against recombinant human cytoglobin and establishment of method for detection of the protein[J]. Journal Of Tropical Medicine, 2011, 11(4): 375-378,400,387,391 |
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| Authors: | TANG Jin-ping WEI Wei WANG Ping LI Zhen LI Ying DONG Wen-qi |
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| Affiliation: | TANG Jin-ping,WEI Wei,WANG Ping,LI Zhen,LI Ying,DONG Wen-qi(School of Biotechnology,Southern Medical University,Guangdong,Guangzhou 510515,China) |
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| Abstract: | Objective To prepare monoclone antibody against recombinant human Cytoglobin(rhCygb) and establish a double antibody sandwich ELISA for the investigation of pharmacokinetic of rhCygb.Methods BALB/c mice were immunized with rhCygb.Methyl cellulose semi-solid culture medium was used for the screening of the hybirdoma cell lines.The characteristics of these monoclonal antibodies were determined by ELISA.A double antibody sandwich ELISA was established.Results Cell lines secreting monoclonal antibody against rh... |
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| Keywords: | recombinant human Cytoglobin monoclonal antibody double antibody sandwich ELISA quantitative detection |
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