| 嗜麦芽窄食单胞菌整合子I和ISCR1研究及ERIC-PCR基因分型 |
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| 引用本文： | 吴奎海,孙静静,陈清,俞守义,王前,芮勇宇.嗜麦芽窄食单胞菌整合子I和ISCR1研究及ERIC-PCR基因分型[J].热带医学杂志,2011,11(4):382-384. |
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| 作者姓名： | 吴奎海 孙静静 陈清 俞守义 王前 芮勇宇 |
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| 作者单位： | 1. 南方医科大学南方医院检验科,广东,广州,510515
2. 南方医科大学公共卫生与热带医学学院流行病学系,广东,广州,510515 |
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| 摘 要： | 目的 了解临床分离嗜麦芽窄食单胞菌的整合子I和ISCR1的分布情况及其基因型.方法 分离临床85株嗜麦芽窄食单胞菌,用WHONET5.4分析菌株药敏情况,采用ERIC-PCR方法 进行基因分型.采用PCR检测整合酶I、整合子I、ISCR1以及ISCR1携带的耐药基因.结果 嗜麦芽窄食单胞菌对亚胺培南、氨曲南、庆大霉素、...
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| 关 键 词： | 嗜麦芽窄食单胞菌 整合子 插入序列共同区1 ERIC-PCR 基因分型 |
Study on class I integron and ISCR1 and molecular epidemiology of Stenotrophomonas maltophilia isolates |
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| WU Kui-hai,SUN Jing-jing,CHEN Qing,YU Shou-yi,WANG Qian,RUI Yong-yu.Study on class I integron and ISCR1 and molecular epidemiology of Stenotrophomonas maltophilia isolates[J].Journal Of Tropical Medicine,2011,11(4):382-384. |
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| Authors: | WU Kui-hai SUN Jing-jing CHEN Qing YU Shou-yi WANG Qian RUI Yong-yu |
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| Institution: | WU Kui-hai1,SUN Jing-jing1,CHEN Qing2,YU Shou-yi2,WANG Qian1,RUI Yong-yu1(1.Department of Clinical Laboratory,Nanfang Hospital,Southern Medical University,Guangdong,Guangzhou 510515,2.Department of Epidemiology School of Public Health and Tropical Medicine,China) |
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| Abstract: | Objective To investigate the expression of class I integron and ISCR1 in Stenotrophomonas maltophilia isolated from clinical species and evaluate the genetic relationship of these isolates..Methods Drug resistance was analyzed by WHONET 5.4.ERIC-PCR was used to determine the genotypes of the isolates.Class I integron,class I integrase,ISCR1 and antibiotic resistance genes carried by ISCR1 were performed by PCR..Results High levels(70%) of resistance to Imipenem,Aztreonam,Gentamicin and Amikacin were found i... |
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| Keywords: | ERIC-PCR |
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